Arf1 regulates the ER-mitochondria encounter structure (ERMES) in a reactive oxygen species-dependent manner
Arf1 regulates the ER-mitochondria encounter structure (ERMES) in a reactive oxygen species-dependent manner
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Arf1 以活性氧依赖性方式调节 ER-线粒体相遇结构 (ERMES)
DOI：
10.1111/febs.14445
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发表时间：
2018
期刊：
影响因子：
5.4
通讯作者：
Mingchun Li
中科院分区：
文献类型：
--
作者：
Bing Zhang;Qilin Yu;Da Huo;Jianrong Li;Chao Liang;Hongyue Li;Xiao Yi;Chenpeng Xiao;Dan Zhang;Mingchun Li
小GTP结合和水解蛋白的Arf家族是膜动力学的一些最重要的细胞内调节剂。在本研究中，我们鉴定了位于高尔基体的Arf家族G蛋白Arf 1，并证实了其在调节分泌途径中的保守功能。有趣的是，ARF 1的缺失导致细胞内活性氧（ROS）的积累，并诱导内质网（ER）-线粒体相遇结构（ERMES）的形成。此外，在arf 1 Δ/Δ菌株中，N-乙酰半胱氨酸介导的ROS清除减弱了ERMES的形成，表明ARF 1缺失导致的细胞内ROS积累促进了ERMES的形成。此外，Arf 1还参与了C.包括细胞周期进程、形态发生和毒力。这项研究揭示了Arf家族G蛋白在调节ERMES形成中的作用，并揭示了ROS对膜动力学的重要贡献。
The Arf family of small GTP‐binding and ‐hydrolyzing proteins are some of the most important intracellular regulators of membrane dynamics. In this study, we identified the Golgi‐localized Arf family G protein Arf1 inCandida albicansand confirmed its conserved function in regulating the secretory pathway. Interestingly, deletion ofARF1resulted in intracellular reactive oxygen species (ROS) accumulation, and induced formation of the endoplasmic reticulum (ER)–mitochondria encounter structure (ERMES). Moreover,N‐acetylcysteine‐mediated ROS scavenging in thearf1Δ/Δ strain attenuated ERMES formation, indicating that intracellular ROS accumulation resulting fromARF1deletion facilitated ERMES formation. In addition, Arf1 regulated many key physiological processes inC. albicans, including cell cycle progression, morphogenesis and virulence. This study uncovers a role for Arf family G proteins in regulating ERMES formation and sheds new light on the crucial contribution of ROS to membrane dynamics.