Taxonomic position and intraspecific variability of the nodule forming Penicillium nodositatum inferred from RFLP analysis of the ribosomal intergenic spacer and Random Amplified Polymorphic DNA
Taxonomic position and intraspecific variability of the nodule forming Penicillium nodositatum inferred from RFLP analysis of the ribosomal intergenic spacer and Random Amplified Polymorphic DNA
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根据核糖体基因间隔区和随机扩增多态性 DNA 的 RFLP 分析推断结节青霉的分类位置和种内变异
DOI：
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
A. Moiroud
中科院分区：
文献类型：
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作者：
Jeanine Sequerra;R. Marmeisse;G. Valla;P. Normand;A. Capellano;A. Moiroud
Penicillium nodositatum 诱导赤杨根部形成根瘤。 PCR 扩增的 ITS1-5·8S-ITS2 核糖体序列中的 RFLP 分析表明，分离出的所有根瘤诱导菌株均属于同质分类群 P. nodositatum，与其他形态相近的青霉属物种不同。从ITS1-5·8S-ITS2 PCR/RFLP数据推断的基因树显示，P. nodositatum序列与属于Biverticillium亚属的Penicillium物种的序列聚类。使用四个 10 聚体引物生成的 RAPD 使我们能够在 10 个不同地理位置收集的 28 个 P. nodositatum 菌株样本中鉴定出四个不同的菌株组。每个采样点仅发现一组。基于 RAPD 的群体与菌株的地理起源之间无法建立联系。然而，我们的结果表明这些群体与赤杨属物种和/或与四种法国赤杨属物种相关的栖息地之间可能存在联系。
Penicillium nodositatum induces the formation of nodules on the roots of Alnus . The analysis of RFLPs in the PCR-amplified ITS1-5·8S-ITS2 ribosomal sequence demonstrates that all the nodule-inducing strains isolated belong to a homogeneous taxon, P. nodositatum , distinct from other morphologically close species of Penicillium . A gene tree inferred from the ITS1-5·8S-ITS2 PCR/RFLP data shows that the P. nodositatum sequence clusters with those of Penicillium species belonging to subgenus Biverticillium . RAPDs generated using four 10-mer primers, allowed us to identify four distinct groups of strains in a sample of 28 strains of P. nodositatum collected in 10 different geographic sites. Only one group was found in each sampled site. No link could be established between RAPD-based groups and the geographic origin of the strains. However, our results suggest a possible link between these groups and Alnus species and/or habitats associated with each of the four French Alnus species.
使用 RAPD PCR 检测已知灵长类谱系后代的非亲本条带过多。
影响因子：
14.9
作者：
Riedy,MF;Hamilton3rd,WJ;Aquadro,CF
通讯作者：
Aquadro,CF