Starvation and re-feeding affect Hsp expression, MAPK activation and antioxidant enzymes activity of European Sea Bass (Dicentrarchus labrax)
Starvation and re-feeding affect Hsp expression, MAPK activation and antioxidant enzymes activity of European Sea Bass (Dicentrarchus labrax)
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DOI：
10.1016/j.cbpa.2013.02.019
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发表时间：
2013-05-01
影响因子：
2.3
通讯作者：
Chatzifotis, Stavros
中科院分区：
文献类型：
--
作者：
Antonopoulou, Efthimia;Kentepozidou, Elissavet;Chatzifotis, Stavros
在鱼类（野生和养殖）食物匮乏的情况下，了解细胞反应是必要的，以便制定战略，以尽量减少饥饿造成的压力，在水产养殖部分。本研究评估了长期饥饿的影响（1F-3S：1个月喂养-3个月饥饿）和饥饿/再喂养（25-2F：两个月饥饿-两个月再喂养）与对照组相比（4F-0S：四个月的喂养-零个月的饥饿）对细胞应激反应和器官中的抗氧化防御的影响，如肠，肝，欧洲海鲈鱼的红色和白色肌肉。分子反应通过Hsp 70和Hsp 90的表达、应激激活蛋白激酶（特别是p38有丝分裂原激活蛋白激酶（p38 MAPK））和细胞外信号调节激酶（ERK-1/2）的磷酸化来解决。为了确定由食物剥夺和/或再喂养引起的氧化应激的影响，研究了抗氧化酶如谷胱甘肽过氧化物酶（GPx）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的（最大）活性以及硫代巴比妥酸反应物质（TBARS）的测定。实验饲养试验引起的组织上的细胞和抗氧化能力的显着和差异的反应，不仅在饥饿的压力过程中，而且在重新喂养的鲈鱼。具体而言，在2S-2F鱼组中，ERK的肠磷酸化和抗氧化酶活性增加，而在1F-35组中，检测到除GPx之外的相同蛋白质的水平增加。在2S-2F鱼的肝脏和红肌中，观察到Hsp 70和磷酸化p38 MAPK水平降低和Hsp 90水平升高。2S-2F和1F-3S组红肌SOD活性降低。1F-3S组肝脏和红肌中Hsp 70水平升高，而肝脏中p38 MAPK活性降低。在白色肌肉中，与对照组相比，在两种喂养方案中，Hsp 90水平降低，p38 MAPK磷酸化增加。在同一组织中，2S-2F处理降低了GPx和过氧化氢酶的水平，而1F-3S处理降低了SOD的水平。(C)2013 Elsevier Inc. All rights reserved.
In the context of food deprivation in fish (wild and farmed), understanding of cellular responses is necessary in order to develop strategies to minimize stress caused by starvation in the aquaculture section. The present study evaluates the effects of long term starvation (1F-3S: one-month feeding-three-month starvation) and starvation/re-feeding (25-2F: two-month starvation-two-month re-feeding) compared to the control group (4F-0S: four-month feeding-zero month starvation) on cellular stress response and antioxidant defense in organs, like the intestine, the liver, the red and white muscle of European sea bass Dicentrarchus labrax. Molecular responses were addressed through the expression of Hsp70 and Hsp90, the phosphorylation of stress-activated protein kinases and particularly p38 mitogen-activated protein kinase (p38 MAPK) and the extracellular signal-regulated kinases (ERK-1/2). For the determination of the effect of the oxidative stress caused by food deprivation and/or re-feeding, the (maximum) activities of antioxidant enzymes such as glutathione peroxidise (GPx), catalase (CAT) and superoxide dismutase (SOD) as well as the determination of thiobarbituric acid reactive substances (TBARS) were studied. The experimental feeding trials caused a tissue distinct and differential response on the cellular and antioxidant capacity of sea bass not only during the stressful process of starvation but also in re-feeding. Specifically, the intestine phosphorylation of ERKs and antioxidant enzymatic activities increased in the 2S-2F fish group, while in the 1F-35 group an increase was detected in the levels of the same proteins except for GPx. In the liver and the red muscle of 2S-2F fish, decreased Hsp70 and phosphorylated p38 MAPK levels and increased Hsp90 levels were observed. Additionally, SOD activity decreased in the red muscle of 2S-2F and 1F-3S groups. In the liver and red muscle of 1F-3S group Hsp70 levels increased, while the activation of p38 MAPK in the liver decreased. In the white muscle, Hsp90 levels decreased and the phosphorylation of p38 MAPK increased in both feeding regimes compared to control. In the same tissue, GPx and catalase levels were decreased in 2S-2F regime, while SOD levels were decreased in 1F-3S regime. (C) 2013 Elsevier Inc. All rights reserved.