Characteristics of synovial fluid required for optimization of lubrication fluid for biotribological experiments
Characteristics of synovial fluid required for optimization of lubrication fluid for biotribological experiments
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DOI：
10.1002/jbm.b.33663
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发表时间：
2017-08-01
影响因子：
3.4
通讯作者：
Gallo, Jiri
中科院分区：
文献类型：
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作者：
Galandakova, Adela;Ulrichova, Jitka;Gallo, Jiri
在将全关节置换（TJR）植入人体之前，在临床前试验中必须进行磨损试验。测试受推荐牛血清（BS）作为润滑液以替代滑液（SF）的现行国际标准管辖。最近，BS的使用受到了批评，因为内容，流体特性和非人类起源的差异。因此，需要更真实的润滑剂模仿SF。为了确定SF的组成，我们分析了在全髋关节和膝关节置换术翻修期间获得的SF，并将其与初次关节置换术期间获得的SF和未进行TJR的患者的SF进行比较。从152名患者中采集样本。我们发现，所有SF的中位总蛋白浓度为36.8 mg/mL，显著高于ISO标准目前推荐的浓度。与未行TJR的患者相比，行TJR翻修的患者的g-球蛋白浓度显著升高，磷脂浓度显著降低。各组透明质酸浓度和粘度无显著差异。我们的研究结果支持需要改进ISO标准中更临床相关的磨损测试润滑剂的定义。(C)2016 Wiley Periodicals，Inc.
Wear testing of total joint replacement (TJR) is mandatory in preclinical testing before implantation of TJR into the human body. Testing is governed by current international standards that recommend bovine serum (BS) as a lubricating fluid to replace synovial fluid (SF). Recently, the use of BS has been criticized because of differences in content, fluid characteristics, and nonhuman origin. As a result, a more realistic lubricant mimicking SF is needed. To define SF composition, we analyzed SF obtained during revisions of total hip and knee arthroplasties and compared it with SF obtained during primary arthroplasties and from patients without TJR. Samples were acquired from 152 patients. We found that the median total protein concentration for all SF was 36.8 mg/mL, which is significantly higher than concentrations currently recommended by the ISO standards. The g-globulin concentration was significantly higher and the phospholipid concentration significantly lower in patients with revision of TJR compared with patients without TJR. No significant difference was found in hyaluronic acid concentration and viscosity among the groups. Our results support the need to improve the definition of a more clinically relevant wear testing lubricant in the ISO standards. (C) 2016 Wiley Periodicals, Inc.