Dynamic Autophagy Map in Mouse Female Germ Cells Throughout the Fetal to Postnatal Life
Dynamic Autophagy Map in Mouse Female Germ Cells Throughout the Fetal to Postnatal Life
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DOI：
10.1007/s43032-022-00940-z
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发表时间：
2022-05
影响因子：
2.9
通讯作者：
Gurong Jiang;Li Zhang;Jiexiang Zhao;Lin Li;Zhenqin Huang;Zhijian Wang
中科院分区：
文献类型：
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作者：
Gurong Jiang;Li Zhang;Jiexiang Zhao;Lin Li;Zhenqin Huang;Zhijian Wang
自噬在小鼠雌性生殖细胞中起着至关重要的作用，但潜在的机制在很大程度上是未知的。在这项研究中，通过查询单细胞RNA-seq数据集，我们研究了自噬相关基因在七种类型生殖细胞（有丝分裂、前瘦素、瘦素、zygotene、粗素、双plotene和dictyate）中的动态表达，并发现了特定阶段的自噬相关基因。利用免疫荧光（IF）和透射电镜（TEM）观察小鼠从有丝分裂到卵泡组装(E12.5（胚胎第12.5天）至P5（出生后第5天）的自噬活性和自噬体数量。此外，通过单样本基因集富集分析（ssGSEA）验证了从E12.5到P5的自噬动力学。我们的研究证明，有丝分裂、二倍体和支配型女性生殖细胞在7种亚型中具有较高的自噬活性。综上所述，我们的工作提供了一个自噬图谱，表明自噬在小鼠雌性生殖细胞从胎儿到出生后的发育过程中是复杂的，这为解读细胞命运转变和女性原发性卵巢功能不全（POI）等不孕问题的自噬调控网络奠定了新的见解。
Autophagy plays vital roles in mouse female germ cells, but the potential mechanism is largely unknown. In this study, by interrogating single-cell RNA-seq dataset, we investigated the dynamic expression of autophagy-related genes in seven types of germ cells (mitosis, pre-leptotene, leptotene, zygotene, pachytene, diplotene, and dictyate) and discovered stage-specific autophagy-related genes. Using immunofluorescence (IF) and transmission electron microscopy (TEM), autophagy activity and autophagosome numbers were revealed from mitosis to follicular assembly (E12.5 (embryonic day 12.5) to P5 (postnatal day 5)). Furthermore, single-sample gene set enrichment analysis (ssGSEA) was performed to validate the autophagy kinetics from E12.5 to P5. Our study proved that the mitosis, diplotene, and dictyate female germ cells had relatively higher autophagy activity among the seven subtypes. In summary, our work provided an autophagy map, suggesting that autophagy was complicated in mouse female germ cell development from the fetal to postnatal life, which paved a new insight for deciphering the autophagy regulatory networks for cell-fate transition and female infertility issues like primary ovarian insufficiency (POI).