Inactivated Mycobacterium phlei inhalation ameliorates allergic asthma through modulating the balance of CD4+CD25+ regulatory T and Th17 cells in mice
Inactivated Mycobacterium phlei inhalation ameliorates allergic asthma through modulating the balance of CD4+CD25+ regulatory T and Th17 cells in mice
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DOI：
10.22038/ijbms.2016.7595
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发表时间：
2016-09
影响因子：
2.2
通讯作者：
Mo-yu Ming;Zhixi Luo;Shengqiu Lv;Qixiang Sun;Chaoqian Li
中科院分区：
文献类型：
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作者：
Mo-yu Ming;Zhixi Luo;Shengqiu Lv;Qixiang Sun;Chaoqian Li
目的：Th2 反应与哮喘的病因有关，但其潜在机制尚不清楚。为了解决这一点，研究了雾化吸入灭活草分枝杆菌对调节哮喘气道炎症的影响。材料与方法：24只雄性BALB/c小鼠随机分为三组：对照组（A组）、哮喘模型组（B组）、药物哮喘模型组（C组）。 B 组和 C 组用卵清蛋白 (OVA) 进行致敏和攻击。 C 组在 OVA 攻击前每天用气溶胶 M. phlei 处理一次。评估每组的气道反应性。处死所有动物，收集肺组织和支气管肺泡灌洗液（BALF）。分析肺组织中的炎症反应、Th17和CD4+CD25+Treg细胞的比例以及细胞因子的水平。结果：B组Th17细胞比例和IL17、IL23、IL23R表达水平升高，Foxp3表达降低。吸入灭活的M. phlei可抑制气道炎症，改善气道高反应性和呼气峰流量（PEF）。此外，C组肺组织中Th17比例、Foxp3水平和CD4+CD25+Treg细胞比例均显着升高。结论：雾化灭活M. phlei可部分通过调节CD4+CD25+调节性T细胞和Th17细胞的平衡来抑制气道炎症和气道高反应性。
Objective(s): Th2 response is related to the aetiology of asthma, but the underlying mechanism is unclear. To address this point, the effect of nebulized inhalation of inactivated Mycobacterium phlei on modulation of asthmatic airway inflammation was investigated. Materials and Methods: 24 male BALB/c mice were randomly divided into three groups: control group (Group A), asthma model group (Group B), and the medicated asthma model group (Group C). Group B and C were sensitized and challenged with ovalbumin (OVA). Group C was treated with aerosol M. phlei once daily before OVA challenge. Airway responsiveness in each group was assessed. All the animals were killed, and lung tissues and bronchoalveolar lavage fluid (BALF) were harvested. Inflammatory response, proportion of Th17 and CD4+CD25+ Treg cells, and the levels of cytokines were analyzed in lung tissue. Results: The proportion of Th17 cells and expression level of IL17, IL23, and IL23R were increased, while Foxp3 expression was decreased in Group B. Inhaling inactivated M. phlei inhibited airway inflammation and improved airway hyper-responsiveness, as well as peak expiratory flow (PEF). In addition, it significantly increased Th17 proportion, Foxp3 level, and the proportion of CD4+CD25+ Treg cells in lung tissue in Group C. Conclusion: Inactivated M. phlei was administered by atomization that suppressed airway inflammation and airway hyper responsiveness partially via modulating the balance of CD4+CD25+ regulatory T and Th17 cells.