Differential immunophenotype of macrophages in acute and chronic chorioamnionitis
Differential immunophenotype of macrophages in acute and chronic chorioamnionitis
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DOI：
10.1515/jpm-2015-0353
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发表时间：
2017-05-01
影响因子：
2.4
通讯作者：
Roh, Cheong-Rae
中科院分区：
文献类型：
--
作者：
Bae, Go-Eun;Honga, Joon-Seok;Roh, Cheong-Rae
目的：本研究的目的是探讨巨噬细胞在急性绒毛膜炎（ACA）和慢性绒毛膜炎（CCA）中的参与和免疫表型，分别标记羊水感染和抗胎儿排斥。方法：绒毛膜羊膜来自（1）无绒毛膜炎的胎龄匹配病例，（2）ACA病例，对3例CCA患者进行CD 14、CD 68、CD 163和DC-SIGN抗体免疫组化染色。结果：与非炎症性病例相比，ACA和CCA病例的绒毛膜滋养层巨噬细胞显著增加。ACA病例蜕膜和绒毛膜羊膜中的巨噬细胞表达CD 14。CCA病例绒毛滋养层巨噬细胞以CD 68阳性为特征。结论：巨噬细胞参与了ACA和CCA的炎症反应。ACA和CCA病例的蜕膜和绒毛膜羊膜中巨噬细胞的差异免疫表型表明其在母胎界面的疾病特异性和区域特异性作用。
Aim: The aim of this study was to investigate the involvement and immunophenotype of macrophages in acute chorioamnionitis (ACA) and chronic chorioamnionitis (CCA), marking amniotic fluid infection and anti-fetal rejection, respectively.Methods: Chorioamniotic membranes from (1) gestational age-matched cases without chorioamnionitis, (2) cases with ACA, and (3) cases with CCA were studied after immunohistochemical staining using antibodies against CD14, CD68, CD163, and DC-SIGN.Results: Macrophages increased prominently in the chorionic trophoblastic layer of both ACA and CCA cases in contrast to non-inflammatory cases. Macrophages in the decidua and the chorioamniotic membranes of ACA cases expressed CD14. Macrophages in the chorionic trophoblastic layer of CCA cases were characterized by CD68 positivity. DC-SIGN-positive cells were increased in the chorioamniotic mesodermal layer of CCA cases.Conclusions: Macrophages participate in the inflammatory response in ACA and CCA. The differential immunophenotypes of macrophages in the decidua and chorioamniotic membranes of ACA and CCA cases suggest their disease-specific and region-specific roles at the fetomaternal interface.