SigN is responsible for differentiation and stress responses based on comparative proteomic analyses of Streptomyces coelicolor wild-type and sigN deletion strains
SigN is responsible for differentiation and stress responses based on comparative proteomic analyses of Streptomyces coelicolor wild-type and sigN deletion strains
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基于天蓝色链霉菌野生型和 sigN 缺失菌株的比较蛋白质组学分析，SigN 负责分化和应激反应。
DOI：
10.1016/j.micres.2009.05.003
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发表时间：
2010-01-01
影响因子：
6.7
通讯作者：
Li, Yongquan
中科院分区：
文献类型：
--
作者：
Wang, Chunxia;Long, Xiaohui;Li, Yongquan
为了研究SigN（SCO 4034）在天蓝色链霉菌（Streptomycescoelicolor）中的功能，我们构建了一个SigN无效突变体M145 Z，该突变体在孢子形成中表现出缺陷。野生型S.通过二维聚丙烯酰胺凝胶电泳（2DPAGE）证明了腔肠色素M145和M145 Z，其鉴定了由于sigN破坏而上调或下调的24个不同的点。其中22个蛋白质通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）鉴定。上调表达的蛋白质涉及能量代谢、胁迫反应、ATP结合、ppGpp严格胁迫反应和转录抗终止等，下调表达的蛋白质与抗生素合成和分化有关。这一结果为研究SigN在S.天蓝色。此外，sigN的删除引起的所有应力条件下，包括热，冷，酸，氧化，盐和乙醇的生长迟缓。这些结果表明SigN参与了S的形态发育、次生代谢和胁迫反应。天蓝色。(C)2009年Elsevier GmbH。All rights reserved.
To address the functions of SigN (SCO4034) in Streptomyces coelicolor, we constructed a sigN null mutant M145Z, which showed a defect in sporulation. The differential proteomic profiles of wild-type S. coelicolors M145 and M145Z were demonstrated by two-dimensional polyacrylamide gel electrophoresis (2D PAGE), which identified 24 different spots that were up- or down-regulated due to sigN disruption. Among them, 22 proteins were identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). The up-regulated proteins were involved in energy metabolism, stress responses, ATP binding, ppGpp stringent stress response and transcriptional anti-termination, etc. The down-regulated proteins were related to antibiotic synthesis and differentiation. The results gave a new insight into the regulatory mechanism of SigN in S. coelicolor. Furthermore, deletion of sigN caused growth retardation under all stress conditions examined including heat, cold, acid, oxidation, salt and ethanol. These results indicated that SigN was involved in morphological development, secondary metabolism and stress responses in S. coelicolor. (C) 2009 Elsevier GmbH. All rights reserved.