TRANSCRIPTIONAL ACTIVATION OF THE MAJOR IMMEDIATE EARLY TRANSCRIPTION UNIT OF HUMAN CYTOMEGALOVIRUS BY HEAT-SHOCK, ARSENITE AND PROTEIN-SYNTHESIS INHIBITORS
TRANSCRIPTIONAL ACTIVATION OF THE MAJOR IMMEDIATE EARLY TRANSCRIPTION UNIT OF HUMAN CYTOMEGALOVIRUS BY HEAT-SHOCK, ARSENITE AND PROTEIN-SYNTHESIS INHIBITORS
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DOI：
10.1099/0022-1317-68-11-2925
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发表时间：
1987-11-01
影响因子：
3.8
通讯作者：
VANDERNOORDAA, J
中科院分区：
文献类型：
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作者：
GEELEN, JLMC;BOOM, R;VANDERNOORDAA, J
在 Rat-9G 细胞中，人类巨细胞病毒 (HCMV) 的主要立即早期 (IE) 转录单位（区域 1 和 2）的多个拷贝稳定整合。这些细胞表现出 IE 表达受抑制的表型，但可以通过抑制蛋白质合成来诱导 IE 表达。在本报告中，我们提供的证据表明，受抑制的表型是由于 IE 转录的缺失造成的，并且热休克和亚砷酸钠处理都会导致受抑制的 IE 转录单元的转录激活。任一处理均导致 HCMV IE 转录物和 IE 核抗原表达的诱导。存在于三个 18 bp IE 增强子元件 (GGACTTTC) 中的八聚体 DNA 序列类似于细胞热休克元件核心共有序列，因此可能参与热休克反应。
In Rat-9G cells several copies of the major immediate early (IE) transcription unit (regions 1 and 2) of the human cytomegalovirus (HCMV) are stable integrated. The cells show a repressed phenotype for IE expression but can be induced by inhibition of protein synthesis. In this report we present evidence that the repressed phenotype is due to the absence of IE transcription and that heat-shock and sodium arsenite treatments each result in the transcriptional activation of the repressed IE transcription unit. Either treatment resulted in the induction of HCMV IE transcripts and IE nuclear antigen expression. An octameric DNA sequence present in three of the 18 bp IE enhancer elements (GGACTTTC) resembles the cellular heat-shock element core consensus sequence and may therefore be involved in the heat-shock response.