Controlled release of sphingosine-1-phosphate agonist with gelatin hydrogels for macrophage recruitment.
Controlled release of sphingosine-1-phosphate agonist with gelatin hydrogels for macrophage recruitment.
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DOI：
10.1016/j.actbio.2014.07.008
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发表时间：
2014-11
影响因子：
9.7
通讯作者：
M. Murakami;Takashi Saito;Y. Tabata
中科院分区：
文献类型：
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作者：
M. Murakami;Takashi Saito;Y. Tabata
本研究的目的是设计一种用于体内促进巨噬细胞募集的药物递送系统（DDS）。作为药物，选择了1-磷酸鞘氨醇1型受体的水不溶性激动剂（SEW 2871）。SEW 2871（SEW）通过与明胶接枝的乳酸低聚物形成胶束而被水增溶。将SEW胶束与明胶混合，然后通过明胶的去水热交联获得包含SEW胶束的明胶水凝胶。SEW从水凝胶中释放，并在体外和体内的SEW胶束。水溶性SEW显示出体外巨噬细胞迁移活性。当植入小鼠背部皮下组织或皮肤伤口缺损时，与不含SEW的水凝胶和与SEW胶束混合的水凝胶相比，含有SEW胶束的水凝胶在很大程度上促进了巨噬细胞向植入部位周围组织的迁移。该水凝胶是一种有前途的DDS，以提高巨噬细胞在体内的招聘。
The objective of this study is to design a drug delivery system (DDS) for the in vivo promotion of macrophage recruitment. As the drug, a water-insoluble agonist of sphingosine-1-phosphate type 1 receptor (SEW2871) was selected. SEW2871 (SEW) was water-solubilized by micelle formation with gelatin grafted byl-lactic acid oligomer. SEW micelles were mixed with gelatin, followed by dehydrothermal crosslinking of gelatin to obtain gelatin hydrogels incorporating SEW micelles. SEW was released from the hydrogels incorporating SEW micelles in vitro and in vivo. The water-solubilized SEW showed in vitro macrophage migration activity. When implanted into the back subcutis or the skin wound defect of mice, the hydrogel incorporating SEW micelles promoted macrophage migration toward the tissue around the implanted site to a significantly great extent compared with SEW-free hydrogel and that mixed with SEW micelles. The hydrogel is a promising DDS to enhance macrophage recruitment in vivo.