The Copper-Controlled RNA Interference System in Trichoderma reesei.
The Copper-Controlled RNA Interference System in Trichoderma reesei.
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DOI：
10.1007/978-1-0716-1048-0_9
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发表时间：
2021
影响因子：
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通讯作者：
Lei Wang;Weixin Zhang;Xiangfeng Meng;Weifeng Liu
中科院分区：
文献类型：
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作者：
Lei Wang;Weixin Zhang;Xiangfeng Meng;Weifeng Liu
里氏木霉能分泌大量的木质纤维素降解酶。虽然T. reesei等的研究成果，但纤维素酶高产的分子机制，包括转录调控和蛋白分泌等尚未完全阐明。这部分是由于缺乏基因操作方法。RNA干扰（RNAi）是真核生物功能基因组研究的有力工具。已经报道了一些成功的RNAi实例;然而，这些系统要么不受控制，要么依赖于营养源诱导型启动子。在这里，我们提出了一个铜控制的RNAi系统在T. reesei不同的靶基因的可逆沉默。作为概念的证明，已经使用这种方法敲低了纤维素酶基因的关键转录激活因子里氏木霉xyr 1。
Trichoderma reeseiis capable of secreting large amounts of lignocellulose-degrading enzymes. Although the genome sequence ofT. reeseihas been available, the molecular mechanisms of the hyper-production of cellulases, including the transcriptional regulation and the protein secretion, have not been completely elucidated yet. This is partially due to the lack of genetic manipulation approaches. RNA interference (RNAi) is a powerful tool for functional genomic studies in eukaryotes. Some successful examples of RNAi have already been reported; however, these systems were either uncontrolled or relied on a nutrient source inducible promoter. Here, we present a copper-controlled RNAi system inT. reeseifor reversible silencing of different target genes. As the proof of concept,T.reesei xyr1, the key transcriptional activator of cellulase genes, has been knocked down using this method.