Alterations of the Gut Microbiota in Response to Total Sleep Deprivation and Recovery Sleep in Rats.
Alterations of the Gut Microbiota in Response to Total Sleep Deprivation and Recovery Sleep in Rats.
复制标题
大鼠完全睡眠剥夺和恢复睡眠时肠道微生物群的变化
DOI：
10.2147/nss.s334985
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发表时间：
2022
影响因子：
3.4
通讯作者：
Chen WH
中科院分区：
文献类型：
--
作者：
Wang Z;Yuan K;Ji YB;Li SX;Shi L;Wang Z;Zhou XY;Bao YP;Xie W;Han Y;Shi J;Lu L;Yan W;Chen WH
越来越多的证据表明，睡眠不足和肠道菌群失调都会导致代谢紊乱。然而，人们对于完全睡眠剥夺（SD）和睡眠恢复对肠道微生物群的组成、功能和代谢动态的影响知之甚少。对无特定病原体的 Sprague-Dawley 大鼠进行 48 小时的 SD 温和处理，然后恢复 1 周。在基线、SD 24 小时、SD 48 小时和恢复 1 周时获得粪便微生物群的分类谱。我们使用 16S rRNA 基因测序来分析大鼠肠道微生物的组成和功能，并进一步表征大鼠体内微生物群衍生的代谢物。微生物群组成分析显示，SD 24小时后，大鼠肠道微生物组成和代谢物没有变化，但SD 48小时后显着改变。这些变化在睡眠恢复 1 周后是可逆的。根据京都基因与基因组百科全书 (KEGG) 注释进行功能分析，表明 SD 大鼠肠道微生物群中的 19 条 KEGG 通路发生了显着改变。这些功能变化发生在SD的24小时内，在SD的48小时后更加明显，并且在睡眠恢复1周后并未完全恢复。这些结果表明，急性总SD会导致肠道微生物群的组成和功能发生显着变化，并且这些变化是可逆的。
Accumulating evidence suggests that both sleep loss and gut dysbiosis can lead to metabolic disorders. However, less is known about the impact of total sleep deprivation (SD) and sleep recovery on the composition, function, and metabolic dynamics of the gut microbiota. Specific-pathogen free Sprague–Dawley rats were subjected to 48 h of SD with gentle handling and then allowed to recover for 1 week. Taxonomic profiles of fecal microbiota were obtained at baseline, 24 h of SD, 48 h of SD, and 1 week of recovery. We used 16S rRNA gene sequencing to analyze the gut microbial composition and function and further characterize microbiota-derived metabolites in rats. The microbiota composition analysis revealed that gut microbial composition and metabolites did not change in the rats after 24 h of SD but were significantly altered after 48 h of SD. These changes were reversible after 1 week of sleep recovery. A functional analysis was performed based on Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations, indicating that 19 KEGG pathways were significantly altered in the gut microbiota in SD rats. These functional changes occurred within 24 h of SD, were more apparent after 48 h of SD, and did not fully recover after 1 week of sleep recovery. These results indicate that acute total SD leads to significant compositional and functional changes in the gut microbiota, and these changes are reversible.