Antibacterial activity of a novel antimicrobial peptide [W7]KR12-KAEK derived from KR-12 against Streptococcus mutans planktonic cells and biofilms
Antibacterial activity of a novel antimicrobial peptide [W7]KR12-KAEK derived from KR-12 against Streptococcus mutans planktonic cells and biofilms
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DOI：
10.1080/08927014.2017.1374378
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发表时间：
2017-01-01
期刊：
影响因子：
2.7
通讯作者：
Teixeira, E. H.
中科院分区：
文献类型：
--
作者：
da Silva, B. R.;Conrado, A. J. S.;Teixeira, E. H.
本研究的目的是描述一种基于KR-12肽一级结构的新型合成肽的合成，并评价其对变形链球菌的抗菌和抗生物膜活性。通过测定最小抑菌浓度（MIC）和最小杀菌浓度（MBC）来评价KR-12和[W-7]KR12-KAEK的抑菌效果。通过总生物量定量、菌落形成单位计数和扫描电镜对其抗生物膜活性进行评价。[W-7]KR12-KAEK的MIC和MBC值分别为31.25 ~ 7.8和62.5 ~ 15.6 μ g ml（-1）。此外，[W-7]KR12-KAEK显著降低了生物膜生物量（50-100%）。在细胞活力方面，[W-7]KR12-KAEK在62.5 ~ 7.8 μ g ml（-1）和500 ~ 62.5 μ g ml（-1）浓度范围内，生物膜形成和预形成的生物膜的cfu数量分别下降。用[W-7]KR12-KAEK处理的变形链球菌的SEM显微照片显示细菌表面有损伤。[W-7]KR12-KAEK被证明是一种控制微生物生物膜的抗菌剂。
The aims of this study were to describe the synthesis of a novel synthetic peptide based on the primary structure of the KR-12 peptide and to evaluate its antimicrobial and anti-biofilm activities against Streptococcus mutans. The antimicrobial effect of KR-12 and [W-7]KR12-KAEK was assessed by determining the minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations. The evaluation of anti-biofilm activity was assessed through total biomass quantification, colony forming unit counting and scanning electron microscopy. [W-7]KR12-KAEK showed MIC and MBC values ranging from 31.25 to 7.8 and 62.5 to 15.6 mu g ml(-1), respectively. Furthermore, [W-7]KR12-KAEK significantly reduced biofilm biomass (50-100%). Regarding cell viability, [W-7]KR12-KAEK showed reductions in the number of CFUs at concentrations ranging from 62.5 to 7.8 mu g ml(-1) and 500 to 62.5 mu g ml(-1) with respect to biofilm formation and preformed biofilms, respectively. SEM micrographs of S. mutans treated with [W-7]KR12-KAEK suggested damage to the bacterial surface. [W-7]KR12-KAEK is demonstrated to be an antimicrobial agent to control microbial biofilms.