A QUANTITATIVE ASSAY FOR NUMBER OF MURINE LYMPHOMA CELLS CAPABLE OF PROLIFERATION IN VIVO
A QUANTITATIVE ASSAY FOR NUMBER OF MURINE LYMPHOMA CELLS CAPABLE OF PROLIFERATION IN VIVO
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DOI：
10.1038/199079a0
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发表时间：
1963-01-01
期刊：
影响因子：
64.8
通讯作者：
VANDERGA.H
中科院分区：
文献类型：
--
作者：
BRUCE, WR;VANDERGA.H
Till、McCulloch和Becker1，2已证明正常小鼠造血组织含有一类细胞，其特征在于它们能够单独在重度辐射受体小鼠的脾脏中产生肉眼可见的集落。菌落计数的简便性使得可以定量研究辐射3、4移植5和红细胞生成6对具有集落形成潜力的细胞数量的影响。这些不同的应用表明，脾集落技术可能会卓有成效地应用于恶性组织的检查。结果表明，小鼠淋巴瘤细胞数量的定量分析能够在体内增殖，确实是可能的。
Till, McCulloch and Becker1,2have demonstrated that normal mouse hæmatopoietic tissue contains a class of cells which are characterized by their ability individually to give rise to macroscopic colonies in the spleens of heavily irradiated recipient mice. The ease with which the colonies may be enumerated has permitted quantitative investigations of the effects of irradiation3,4transplantation5, and erythropoiesis6on the number of cells with colony-forming potentiality. These varied applications suggested to us that the spleen-colony technique might be fruitfully applied to the examination of malignant tissue. The results show that a quantitative assay of the number of murine lymphoma cells capable of proliferatingin vivois, indeed, possible.