Changes of the CYP11B2 expressing zona glomerulosa in human adrenals from birth to 40 years of age
Changes of the CYP11B2 expressing zona glomerulosa in human adrenals from birth to 40 years of age
复制标题
人肾上腺表达CYP11B2的肾小球带从出生到40岁的变化
DOI：
10.1161/hypertensionaha.122.19052
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发表时间：
2022
期刊：
影响因子：
8.3
通讯作者：
J.
中科院分区：
文献类型：
--
作者：
van de Wiel;E.;Baz;A.-H. C.;Kusters;B.;Mukai;K.;van Bonzel;L.;van Erp;M.;Deinum;J.;Langenhuijsen;J.
研究背景免疫组织化学中的醛固酮合成酶抗体(CYP11B2)可用于显示产醛固酮的球状带(ZG)、产醛固酮的小结和产醛固酮的腺瘤。ZG的架构在年老和年少时有所不同，但变化的演变并不是很清楚。产生醛固酮的微小结节和产生醛固酮的腺瘤的发病机制仍然不清楚，对年轻人群中ZG的研究也是有限的。在这项研究中，我们通过量化CYP11B2的表达来阐明人类ZG随年龄的变化。方法从57例0-40岁的尸检病例中收集83个人肾上腺。在26例中，双侧肾上腺均可用。结果年龄与细胞色素P11B2的相对表达面积呈负相关，与细胞色素P11B2的连续性呈负相关，与平均间隙长度呈正相关，与细胞色素P11B2的最大表达深度呈正相关。结论ZG相对表达面积和连续性的下降可能预示着ZG的退缩，间隙的增加和最大深度指示聚集，相当于产生醛固酮的微结节的形成。同一病例的两个肾上腺的相似之处表明，这些变化发生在两侧。
BackgroundAldosterone synthase (CYP11B2) antibodies for immunohistochemistry, enables to visualize aldosterone-producing zona glomerulosa (ZG), aldosterone-producing micronodules, and aldosterone-producing adenomas. The architecture of the ZG differs in old versus young age but the evolution of the changes is not well known. The pathogenesis of aldosterone-producing micronodules and aldosterone-producing adenomas is still unclear and research on the ZG in young populations is limited. In this study, we elucidate changes in human ZG with age by quantifying the CYP11B2 expression.MethodsWe collected 83 human adrenal glands from 57 autopsy cases aged 0 to 40 years old. In 26 cases, both adrenals were available. We performed immunohistochemistry targeting CYP11B2 and quantified the relative CYP11B2 expressing area, CYP11B2 continuity, the mean gap length between CYP11B2-expressing areas and the maximum extension of CYP11B2 area (depth).ResultsWe found a negative correlation between age and the relative CYP11B2 expressing area, a negative correlation between age and CYP11B2 continuity, a positive correlation between age and mean gap length, and a positive correlation between age and maximum CYP11B2 depth. The changes in expression patterns of relative CYP11B2 expressing area, CYP11B2 continuity and mean gap length were seen in both adrenals of the same autopsy case.ConclusionsThe decline of relative CYP11B2 expressing ZG area and continuity may indicate involution of the ZG, which is supported by an increase of gaps and maximum CYP11B2 depth indicating clustering, comparable to formation of aldosterone-producing micronodules. The similarities in both adrenals from the same case indicate that these changes occur bilaterally.