Polydiacetylenyl β-cyclodextrin based smart vesicles for colorimetric assay of arginine and lysine.
Polydiacetylenyl β-cyclodextrin based smart vesicles for colorimetric assay of arginine and lysine.
复制标题
DOI：
10.1038/srep31115
复制
发表时间：
2016-08-09
影响因子：
4.6
通讯作者：
Jung S
中科院分区：
文献类型：
--
作者：
Cho E;Kim H;Choi Y;Paik SR;Jung S
Selective visualization of arginine and lysine has been explored among 20 amino acids using the hybrid conjugate of β-cyclodextrin (β-CD) and polydiacetylene (PDA). The mono pentacosa-10,12-diynyl aminomethyl group was successfully coupled to either the primary or the secondary face of β-CD, where mono-6-amino-6-deoxy-β-CD or mono-3-amino-3-deoxy-β-CD reacted with the N-hydroxysuccinimide ester of 10,12-pentacosadiynoic acid. In this combinatorial system, the cylindrical β-cyclodextrin functions as a channel for the introduction of the cationic amino acids to the artificial membrane. The membrane perturbation and aggregation by the target amino acids could be exclusively visualized as a blue to red color change based on the responsive polydiacetylene domain. These interesting findings demonstrated that the developed β-CD conjugated PDA system may offer a new method of cell-penetrating mechanism, a promising vector system, as well as impact the production industry of arginine or lysine.
