Comparison of cannabinoid concentrations in oral fluid and whole blood between occasional and regular cannabis smokers prior to and after smoking a cannabis joint
Comparison of cannabinoid concentrations in oral fluid and whole blood between occasional and regular cannabis smokers prior to and after smoking a cannabis joint
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DOI：
10.1007/s00216-013-7412-1
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发表时间：
2013-12-01
影响因子：
4.3
通讯作者：
Giroud, Christian
中科院分区：
文献类型：
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作者：
Fabritius, Marie;Chtioui, Haithem;Giroud, Christian
对偶尔吸烟者和重度吸烟者进行了一项交叉对照研究。参与者在两个不同的场合吸食大麻（11%的δ 9-四氢大麻酚（THC））或相应的安慰剂。在吸烟前和吸烟后3.5 h内采集全血和口服液样本。根据这些数据进行药代动力学分析。评估主观影响的调查问卷在吸烟前后的每个疗程中发给受试者。采用气相色谱(LC)-串联质谱（MS/MS）分析血液中四氢大麻酚（THC）、11-羟基四氢大麻酚（11-OH-THC）和11-不-9-羧基四氢大麻酚（THCCOOH）。四氢大麻酚（THC）、四氢大麻酚（thcooh）、大麻酚（CBN）和δ 9-四氢大麻酚酸A （THC-A）在of上仅采用LC-MS/MS进行测定。根据吸食大麻会对口腔造成严重污染的普遍假设，我们发现，在吸食大麻后，四氢大麻酚和四氢大麻酚a浓度出现一个急剧的高浓度峰值，在3小时内迅速下降。两组之间的四氢大麻酚血液和of中值最大浓度没有明显差异；在偶尔吸烟者和重度吸烟者中，这些水平分别等于1338和1041 μ g/L的OF和82和94 μ g/L的WB。经常吸烟者的初始WB THCCOOH浓度比偶尔吸烟者高得多。与偶尔吸烟者相比，经常吸烟者的迷糊感要少得多，而陶醉感几乎没有变化。
A cross-over controlled administration study of smoked cannabis was carried out on occasional and heavy smokers. The participants smoked a joint (11 % Delta 9-tetrahydrocannabinol (THC)) or a matching placebo on two different occasions. Whole blood (WB) and oral fluid (OF) samples were collected before and up to 3.5 h after smoking the joints. Pharmacokinetic analyses were obtained from these data. Questionnaires assessing the subjective effects were administered to the subjects during each session before and after the smoking time period. THC, 11-hydroxy-THC (11-OH-THC) and 11-nor-9-carboxy-THC (THCCOOH) were analyzed in the blood by gas chromatography or liquid chromatography (LC)-tandem mass spectrometry (MS/MS). The determination of THC, THCCOOH, cannabinol (CBN), and Delta 9-tetrahydrocannabinolic acid A (THC-A) was carried out on OF only using LC-MS/MS. In line with the widely accepted assumption that cannabis smoking results in a strong contamination of the oral cavity, we found that THC, and also THC-A, shows a sharp, high concentration peak just after smoking, with a rapid decrease in these levels within 3 h. No obvious differences were found between both groups concerning THC median maximum concentrations measured either in blood or in OF; these levels were equal to 1,338 and 1,041 mu g/L in OF and to 82 and 94 mu g/L in WB for occasional and heavy smokers, respectively. The initial WB THCCOOH concentration was much higher in regular smokers than in occasional users. Compared with the occasional smokers, the sensation of confusion felt by the regular smokers was much less while the feeling of intoxication remained almost unchanged.