Novel transcription regulatory elements in Caenorhabditis elegans muscle genes
Novel transcription regulatory elements in Caenorhabditis elegans muscle genes
复制标题
DOI：
10.1101/gr.2961104
复制
发表时间：
2004-12-01
期刊：
影响因子：
7
通讯作者：
Stormo, GD
中科院分区：
文献类型：
--
作者：
GuhaThakurta, D;Schriefer, LA;Stormo, GD
我们报道了在秀丽隐杆线虫中与肌肉基因表达相关的三个新的转录调控元件的鉴定。从表征良好的线虫肌肉基因子集开始，我们使用计算DNA模式识别算法在启动子区域确定了保守的DNA基序。这些被认为是假定的肌肉转录调控基序。以绿色荧光蛋白（GFP）为报告蛋白，实验确定了这些基序在驱动肌肉基因表达中的生物活性。基于这三个基序的肌肉表达预测精度令人鼓舞；10个先前预测有肌肉表达的未表征基因中有9个被证明在肌肉组织中特异性或选择性地表达，而在肌肉中表达这些基序的9个基因中只有1个得分较低。mlc-2和unc-89基因启动子中可能的调控元件敲除表明它们显著促进肌肉表达，并以协同方式起作用。我们发现这些DNA基序也存在于C， briggsae的肌肉启动子中，表明它们在线虫中具有功能保守性。
We report the identification of three new transcription regulatory elements that are associated with muscle gene expression in the nematode Caenorhabditis elegans. Starting from a subset of well-characterized nematode muscle genes, we identified conserved DNA motifs in the promoter regions using computational DNA pattern-recognition algorithms. These were considered to be putative muscle transcription regulatory motifs. Using the green-fluorescent protein (GFP) as a reporter, experiments were done to determine the biological activity of these motifs in driving muscle gene expression. Prediction accuracy of muscle expression based on the presence of these three motifs was encouraging; nine of 10 previously uncharacterized genes that were predicted to have muscle expression were shown to be expressed either specifically or selectively in the muscle tissues, whereas only one of the nine that scored low for these motifs expressed in muscle. Knockouts of putative regulatory elements in the promoter of the mlc-2 and unc-89 genes show that they significantly contribute to muscle expression and act in a synergistic manner. We find that these DNA motifs are also present in the muscle promoters of C, briggsae, indicating that they are functionally conserved in the nematodes.