Intra- and Interskeletal Proteome Variations in Fresh and Buried Bones
Intra- and Interskeletal Proteome Variations in Fresh and Buried Bones
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DOI：
10.1021/acs.jproteome.6b01070
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发表时间：
2017-05-01
影响因子：
4.4
通讯作者：
Buckley, Michael
中科院分区：
文献类型：
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作者：
Procopio, Noemi;Chamberlain, Andrew T.;Buckley, Michael
由于骨骼遗骸中蛋白质的寿命，蛋白质组学方法在考古学和古生物学中越来越重要。法医学应用的兴趣也在不断发展，有可能揭示死后间隔和死亡年龄估计。然而，我们的理解内和骨骼间的蛋白质组的变化是目前严重有限的。在这里，我们评估了从五个不同的子样本的不同骨骼元素从埋葬的猪尸体，以确定个体内和个体之间的变化程度。我们发现，数据的可重复性取决于用于采样的骨骼元素，骨内差异超过从不同个体采样的相同骨骼元素之间观察到的差异。有趣的是，几种血清蛋白的丰度似乎与生物年龄相关，α-1抗胰蛋白酶和嗜铬粒蛋白-A的相对浓度增加，胎球蛋白-A的相对浓度降低。我们还观察到来自不同LC-MS/MS运行的数据在相隔数月分析的类似样品的等分试样上的惊人水平的差异，这对不同研究中此类方法的结果比较增加了限制。
Proteomic methods are acquiring greater importance in archaeology and palaeontology due to the longevity of proteins in skeletal remains. There are also developing interests in forensic applications, offering the potential to shed light on post-mortem intervals and age at death estimation. However, our understanding of intra- and interskeletal proteome variations is currently severely limited. Here, we evaluated the proteomes obtained from five distinct subsamples of different skeletal elements from buried pig carcasses to ascertain the extent of variation within and between individuals. We found that reproducibility of data depends on the skeletal element used for sampling and that intrabone differences exceed those observed between the same skeletal element sampled from different individuals. Interestingly, the abundance of several serum proteins appeared to correlate with biological age with relative concentrations of alpha-1 antitrypsin and chromogranin-A increasing and those of fetuin-A decreasing. We also observed a surprising level of divergence in data from different LC-MS/MS runs on aliquots of similar samples analyzed months apart, adding constraints to the comparison of results of such methods across different studies.