Effects of Abscisic Acid on Antioxidant Systems of Stylosanthes guianensis (Aublet) Sw. under Chilling Stress
Effects of Abscisic Acid on Antioxidant Systems of Stylosanthes guianensis (Aublet) Sw. under Chilling Stress
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DOI：
10.2135/cropsci2005.0599
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发表时间：
2005-03
期刊：
影响因子：
2.3
通讯作者：
Biyan Zhou;Zhenfei Guo;Zhi-li Liu
中科院分区：
文献类型：
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作者：
Biyan Zhou;Zhenfei Guo;Zhi-li Liu
柱花草Stylosanthes guianensis（Aublet）Sw.是热带、亚热带国家重要的牧草。寒害S.圭亚那线虫是亚热带栽培区的一个严重问题。在控制条件下，研究了脱落酸（阿坝）对S。低温胁迫下的抗氧化系统柱花草幼苗用10 mg L（-1）阿坝或水进行喷雾处理。一天后，将植物转移到10摄氏度的生长室中，并在160微摩尔m（-2）s（-1）光合光子通量密度下以12小时的光周期生长7天。然后将冷却处理的植物再加热至28 ℃ 2天。在9天的治疗，一系列的酶活性，相对含水量（RWC），和电解质渗漏进行了测量取样小叶。结果表明，低温处理增加了水处理和ABA处理植株的电解质渗漏，而RWC在低温条件下下降。ABA处理的植株比清水处理的植株具有更低的电解质渗漏和更高的RWC。在低温胁迫前，阿坝处理可短暂提高抗坏血酸过氧化物酶（APX）和过氧化氢酶（CAT）的活性以及还原型谷胱甘肽（GSH）和抗坏血酸（阿萨）的含量。ABA处理的S.在低温条件下，与清水处理相比，水处理的圭亚那菜保留了更高水平的超氧化物歧化酶（SOD）、APX、GSH和阿萨。结果表明，ABA-1提高了S. guianensis部分与增强的清除系统有关。
Stylosanthes guianensis (Aublet) Sw. is an important pasture legume in tropical and subtropical countries. Chilling injury of S. guianensis is a serious problem in subtropical cultivated areas. An experiment was conducted under controlled conditions to study the effect of abscisic acid (ABA) on S. guianensis and its relation to antioxidant systems under chilling stress. Stylosanthes guianensis seedlings were sprayed with 10 mg L(-1) ABA or water. One day later, the plants were transferred to a 10 degrees C growth chamber and grown for 7 d with a 12-h photoperiod at 160 micromol m(-2) s(-1) photosynthetic photon flux density. The chilling treated plants were then rewarmed to 28 degrees C for 2 d. During the 9-d treatment, a series of enzyme activities, relative water content (RWC), and electrolyte leakage were measured on sampled leaflets. The results showed that chilling increased electrolyte leakage of both water- and ABA-treated plants, while RWC decreased under chilling conditions. ABA-treated plants had lower electrolyte leakage and higher RWC than those of water-treated plants. Activities of ascorbate peroxidase (APX) and catalase (CAT) and contents of reduced glutathione (GSH) and ascorbic acid (AsA) were transiently enhanced by ABA treatment before the plants were subject to chilling. ABA-treated S. guianensis retained higher levels of superoxide dismutase (SOD), APX, GSH, and AsA than water-treated ones under chilling conditions. The results suggested that ABA-increased chilling resistance in S. guianensis is partially associated with enhanced scavenging systems.