Effect of vitamin C depletion on UVR-B induced cataract in SMP30/GNL knockout mice
Effect of vitamin C depletion on UVR-B induced cataract in SMP30/GNL knockout mice
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DOI：
10.1016/j.exer.2011.11.010
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发表时间：
2012-01-01
影响因子：
3.4
通讯作者：
Kurosaka, Daijiro
中科院分区：
文献类型：
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作者：
Ishikawa, Yohei;Hashizume, Kouhei;Kurosaka, Daijiro
我们研究了小鼠模型中维生素 C (VC) 减少是否会增加体内暴露于 B 型紫外线辐射 (UVR-B) 引起的晶状体混浊（白内障）。衰老标记蛋白 30 (SMP30) 敲除 (1(0) 小鼠由于葡萄糖酸内酯酶 (GNL) 基因的遗传破坏而无法合成 VC，被分为 2 组： VC充足（VC（+））和VC不足（VC（-））。从 1 个月大开始，这些组可以自由饮用分别含有 0.0375 和 1.5 g/L VC 的水。 SMP30 KO VC (-)、SMP30 KO VC (+) 和野生型 (WT) 小鼠均为 14 周龄，单侧体内暴露于 UVR-B（200 mW/cm(2))，持续 100 秒，每周两次，持续 3 周（总计：1200 mJ/cm(2)）。最后一次UVR-B照射后48小时，记录白内障形态，并将白内障诱发的比率量化为白内障面积比（不透明面积/前囊）。在SMP30 KO中，UVR-B照射主要在前囊下诱发白内障 VC (-)、SMP30 KO VC (+) 和 WT 小鼠。在 SMP30 KO VC (-) 镜片中，混浊范围比 SMP30 KO VC (+) 或 WT 镜片更广泛（白内障面积比：59.3% +/- 10% 对比 32.2% +/- 11.7% 或 29.0% +/- 9.0%；P < 0.01）。 总之，VC 在无法内源性产生 VC 的小鼠中，缺乏可能会增加 UVR-B 诱发白内障的易感性。 (C) 2011 Elsevier Ltd. 保留所有权利。
We investigated whether decreased vitamin C (VC) in a mouse model increases lens opacity (cataract) induced by in vivo exposure to ultraviolet radiation type B (UVR-B).Senescence marker protein-30 (SMP30) knockout (1(0) mice, which cannot synthesize VC due to genetic disruption of the gluconolactonase (GNL) gene, were divided into 2 groups: VC sufficient (VC (+)) and VC deficient (VC (-)). Starting at 1 month of age, these groups had free access to water containing 0.0375 and 1.5 g/L of VC, respectively. SMP30 KO VC (-), SMP30 KO VC (+), and wild-type (WT) mice, all 14 weeks of age, were unilaterally exposed in vivo to UVR-B (200 mW/cm(2)) for 100 s twice a week for 3 weeks (total: 1200 mJ/cm(2)). At 48 h after the last UVR-B exposure, cataract morphology was documented, and the ratio of cataract induction was quantified as the cataract area ratio (opacity area/anterior capsule).UVR-B exposure induced cataract mainly at anterior sub-capsular in SMP30 KO VC (-), SMP30 KO VC (+), and WT mice. In SMP30 KO VC (-) lenses the opacities were more extensive than in SMP30 KO VC (+) or WT lenses (cataract area ratios: 59.3% +/- 10% vs. 32.2% +/- 11.7% or 29.0% +/- 9.0%; P < 0.01).In conclusion, VC depletion may increase the susceptibility to develop UVR-B induced cataracts in mice unable to endogenously produce VC. (C) 2011 Elsevier Ltd. All rights reserved.