Adhesion and Bactericidal Properties of a Wettability-Controlled Artificial Nanostructure
Adhesion and Bactericidal Properties of a Wettability-Controlled Artificial Nanostructure
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DOI：
10.1021/acsanm.8b01340
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发表时间：
2018-10-01
影响因子：
5.9
通讯作者：
Ito, Takeshi
中科院分区：
文献类型：
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作者：
Nakade, Kazuki;Jindai, Keisuke;Ito, Takeshi
利用大肠杆菌评价了硅纳米柱阵列的纳米结构表面的粘附行为和杀菌性能。通过自组装单层膜（SAM）控制纳米结构表面的润湿。粘附行为强烈地依赖于表面的润湿性以及它是纳米结构的还是平坦的表面。在纳米结构表面上的粘附细胞的数量高于在平坦的Au表面上的粘附细胞的数量。此外，在纳米结构表面上，细胞膜在较高接触角下比在较低接触角下受到更强烈的破坏。使用延时成像来分析由细胞间液（包括荧光蛋白（mCherry））的渗出引起的荧光强度的变化，所述细胞间液在单细胞水平上位于机翼表面和人造纳米结构表面上。我们发现，有三个阶段的荧光强度梯度的变化。
The adhesion behavior and bactericidal properties of the nanostructured surface of Si nanopillar array, which mimicked a cicada wing surface, were evaluated using Escherichia coli. Wettability of the nanostructured surface was controlled by using self-assembled monolayer (SAM). The adhesion behavior was strongly dependent on the wettability of the surface and whether it was a nanostructured or a flat surface. The number of adhered cells on the nanostructured surface was higher than that on the flat Au surface. In addition, the cell membrane was more strongly damaged at a higher contact angle than at a lower contact angle on the nanostructured surface. Time-lapse imaging was used to analyze the changes in fluorescence intensity caused by the effusion of an intercellular fluid, including fluorescent protein (mCherry), at the single cell level on the cicada wing surface and the artificial nanostructured surface. We found that there were three stages of changes in the fluorescence intensity gradient.