AUTOMATED CHEMICAL SYNTHESIS OF A PROTEIN-GROWTH FACTOR FOR HEMATOPOIETIC-CELLS, INTERLEUKIN-3
AUTOMATED CHEMICAL SYNTHESIS OF A PROTEIN-GROWTH FACTOR FOR HEMATOPOIETIC-CELLS, INTERLEUKIN-3
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DOI：
10.1126/science.3079915
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发表时间：
1986-01-10
期刊：
影响因子：
56.9
通讯作者：
KENT, SBH
中科院分区：
文献类型：
--
作者：
CLARKLEWIS, I;AEBERSOLD, R;KENT, SBH
白细胞介素-3（IL-3）是一种由140个氨基酸组成的蛋白质，通过自动肽合成仪进行化学合成，并显示具有天然IL-3的生物活性。合成类似物的测定确定氨基末端片段具有可检测的IL-3活性，但完整分子的稳定三级结构是完全活性所必需的。结果表明，自动化肽合成可以应用于蛋白质的结构和功能的研究。
Interleukin-3 (IL-3), a protein of 140 amino acids, was chemically synthesized by means of an automated peptide synthesizer and was shown to have the biological activities attributed to native IL-3. Assays of synthetic analogues established that an amino terminal fragment has detectable IL-3 activity, but that the stable tertiary structure of the complete molecule was required for full activity. The results demonstrate that automated peptide synthesis can be applied to the study of the structure and function of proteins.