Acrosome reaction of mouse epididymal sperm on oocyte zona pellucida
Acrosome reaction of mouse epididymal sperm on oocyte zona pellucida
复制标题
DOI：
10.1262/jrd.18145
复制
发表时间：
2007-04-01
影响因子：
1.8
通讯作者：
Baba, Tadashi
中科院分区：
文献类型：
--
作者：
Yamashita, Misuzu;Yamagata, Kazuo;Baba, Tadashi
为了提高小鼠附睾精子顶体反应的评估，我们采用抗Izumo 1抗体代替顶体蛋白抗体。顶体完整，自发顶体反应，真正的顶体反应，可能死亡和/或膜损伤的精子之间的顶体状态明确区分抗Izumo 1抗体，DNA染料Hoechst 33342，和单克隆抗体MN 7多聚甲醛固定的精子的联合应用。当用抗Izumo 1抗体检测获能附睾精子对卵母细胞透明质膜的顶体反应时，受精后30分钟，约20%的精子结合在透明质膜上进行顶体反应。我们还观察到的时刻，顶体反应的活精子上的透明质酸通过延时监测使用异硫氰酸荧光素标记的抗Izumo 1抗体。
To improve assessment of the acrosome reaction of mouse epididymal sperm, we employed anti-Izumo1 antibody instead of antibodies against acrosomal proteins. The acrosomal states among acrosome-intact, spontaneously acrosome-reacted, truly acrosome-reacted, and probably dead and/or membrane-damaged sperm were clearly distinguished by combined application of anti-Izumo1 antibody, DNA dye Hoechst 33342, and monoclonal antibody MN7 to paraformaldehyde-fixed sperm. When the acrosome reaction of capacitated epididymal sperm on the oocyte zona pellucida was examined using anti-Izumo1 antibody, approximately 20% of sperm bound onto the zona pellucida were acrosome-reacted 30 min after insemination. We also observed the moment of the acrosome reaction of live sperm on the zona pellucida by time-lapse monitoring using fluorescein isothiocyanate-conjugated anti-Izumo1 antibody.