Molecular characterization of Ramie mosaic virus isolates detected in Jiangsu and Zhejiang provinces, China
Molecular characterization of Ramie mosaic virus isolates detected in Jiangsu and Zhejiang provinces, China
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中国江苏省和浙江省检测到的苎麻花叶病毒分离株的分子特征
DOI：
10.4149/av_2010_03_225
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发表时间：
2010-01-01
期刊：
影响因子：
1.7
通讯作者：
Qian, Y. J.
中科院分区：
文献类型：
--
作者：
Li, J.;Zhang, X. Y.;Qian, Y. J.
从苎麻（Boehmeria nivea L.）表现黄色花叶症状，采自中国江苏省和浙江省。用菜豆花叶病毒通用引物PA/PB扩增出的部分DNA-A片段序列比较表明，这些病毒分离物具有较高的序列同源性。J 4和Z1株的DNA-A全序列分别为2736和2737 nt，核苷酸序列同源性为94.7%。此外，DNA-B组分被鉴定为J 4和Z1分离物，分别包含2717和2719个核苷酸，彼此共享88.6%的核苷酸序列同一性。序列比对和系统发育分析表明，J 4和Z1分离物与苎麻花叶病毒（RamMV）分离物的DNA-A序列同源性最高（93.6-94.7%）。这些分子生物学数据表明，J 4和Z1可能是RamMV的两个不同分离物。这是首次报道在中国这些地区发生双分型菜豆花叶病毒。
Virus isolates were obtained from three ramie samples (Boehmeria nivea L.) showing yellow mosaic symptoms collected in Jiangsu and Zhejiang provinces, China. Comparison of partial DNA-A fragments amplified with begomovirus universal primers PA/PB revealed that these viral isolates shared a high sequence identity. The complete DNA-A sequences of two isolates J4 and Z1 were determined to be 2736 and 2737 nts, respectively, sharing 94.7% nucleotide sequence identity with each other. Also, the DNA-B components were identified for J4 and Z1 isolates and comprised 2717 and 2719 nts, respectively, sharing 88.6% nucleotide sequence identity with each other. Furthermore, sequence alignment and phylogenetic analysis showed that J4 and Z1 isolates had the highest sequence identities (93.6-94.7%) with isolates of Ramie mosaic virus (RamMV) for DNA-A. These molecular data suggested that J4 and Z1 may be two different isolates of RamMV. This is the first report about the occurrence of a bipartite begomovirus in these regions of China.