Human BRCA2 protein promotes RAD51 filament formation on RPA-covered single-stranded DNA
Human BRCA2 protein promotes RAD51 filament formation on RPA-covered single-stranded DNA
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DOI：
10.1038/nsmb.1904
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发表时间：
2010-10-01
影响因子：
16.8
通讯作者：
Heyer, Wolf-Dietrich
中科院分区：
文献类型：
--
作者：
Liu, Jie;Doty, Tammy;Heyer, Wolf-Dietrich
BRCA2是一种肿瘤抑制因子，参与同源重组，这是基因组完整性的关键途径。BRCA2与重组的中心蛋白RAD51相互作用，在单链DNA(SsDNA)上形成丝状结构，进行同源性搜索和DNA链侵袭。我们报道了全长人BRCA2的纯化，结果表明，它与6个类似的RAD51分子结合，并以DSS1刺激的方式促进RAD51与复制蛋白A(RPA)包被的ssDNA结合。
BRCA2 is a tumor suppressor that functions in homologous recombination, a key genomic integrity pathway. BRCA2 interacts with RAD51, the central protein of recombination, which forms filaments on single-stranded DNA (ssDNA) to perform homology search and DNA strand invasion. We report the purification of full-length human BRCA2 and show that it binds to similar to 6 RAD51 molecules and promotes RAD51 binding to ssDNA coated by replication protein A (RPA), in a manner that is stimulated by DSS1.