A historical and modern perspective on plant cytogenetics
A historical and modern perspective on plant cytogenetics
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DOI：
10.1093/bfgp/elp058
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发表时间：
2010-03-01
影响因子：
4
通讯作者：
Bass, Hank W.
中科院分区：
文献类型：
--
作者：
Figueroa, Debbie M.;Bass, Hank W.
植物细胞遗传学继续蓬勃发展，并通过描绘标记顺序，定义重叠群缺口和揭示基因组重排对基因组学项目做出了重要贡献。在这里，我们回顾了植物细胞遗传学的概念，通过分子生物学和基因组学的时代。染色体制备的重大进展，如扩展光纤FISH，大大提高了轴向分辨率的限制，而成像和信号放大技术提高了我们检测小基因大小探针的能力。将传统的FISH技术与染色质免疫细胞化学相结合，有助于拓宽植物细胞遗传学的能力，从而阐明基因组的结构和组织。这些进展进行了描述，连同选定的例子，说明在指导基因组计划的植物细胞遗传学的力量。
Plant cytogenetics has continued to flourish and make essential contributions to genomics projects by delineating marker order, defining contig gaps and revealing genome rearrangements. Here we review the field of plant cytogenetics from its conception through the eras of molecular biology and genomics. Significant advances in chromosome preparation, such as extended fiber-FISH, have greatly increased the axial resolution limits, while imaging and signal amplification technologies have improved our ability to detect small gene-sized probes. Combinations of traditional FISH technologies with chromatin immunocytochemistry serve to broaden the ability of plant cytogenetics to shed light on genome structure and organization. These advances are described, together with selected examples that illustrate the power of plant cytogenetics in guiding genome projects.