Circulating Levels of Soluble α-Klotho Are Markedly Elevated in Human Umbilical Cord Blood
Circulating Levels of Soluble α-Klotho Are Markedly Elevated in Human Umbilical Cord Blood
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DOI：
10.1210/jc.2010-2357
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发表时间：
2011-06-01
影响因子：
5.8
通讯作者：
Ozono, Keiichi
中科院分区：
文献类型：
--
作者：
Ohata, Yasuhisa;Arahori, Hitomi;Ozono, Keiichi
背景：胎儿血清钙、磷水平高于母体水平。目的：本研究旨在测定胎儿中可溶性α-Klotho的基线水平，并将其与新生儿、母亲和成人的水平进行比较，以阐明α-Klotho是否参与了对钙和磷代谢的胎儿特异性调节。设计和背景：我们对一家医院的健康婴儿(出生时和/或出生后4天)、他们的母亲和成年志愿者进行了一项横断面评估。参与者：21名健康母亲及其婴儿(共23名，结果：脐带血中α-Klotho水平(3243+/-1899 pg/ml)明显高于出生4 d的新生儿(582+/-90 pg/ml)、母亲(768+/-261 pg/ml)和成年志愿者(681+/-140 pg/ml)(P<；0.001)，而胎儿FGF23水平低于其他受试者。脐血可溶性α-Klotho水平与FGF23水平呈负相关。免疫组织化学结果显示，α-Klotho在正常足月胎盘合体滋养层细胞中主要表达。结论：脐血中可溶性α-Klotho水平显著升高，可作为胎儿矿物质代谢的生物标志物。(J临床内分泌Metab 96：E943-E947,2011)
Context: Fetal serum levels of calcium and phosphate are higher than those in the maternal levels. Although alpha-Klotho is known to participate in calcium and phosphate metabolism in adults, its role in the perinatal period remains unknown.Objective: This study aimed to determine the baseline levels of soluble alpha-Klotho in fetuses and compare them with those in neonates, mothers, and adults to clarify whether alpha-Klotho is involved in the fetal-specific regulation of calcium and phosphate metabolism.Design and Setting: We conducted a cross-sectional evaluation of healthy babies (at birth and/or at 4 d after birth), their mothers, and adult volunteers at one hospital.Participants: Twenty-one healthy mothers, their babies (23 in total, including two pairs of twins), and 25 adult volunteers participated in the study.Main Outcome Measures: We measured the serum levels of soluble alpha-Klotho and fibroblast growth factor 23 (FGF23).Results: In cord blood, the level of alpha-Klotho was markedly higher (3243 +/- 1899 pg/ml) than levels in neonates at d 4 (582 +/- 90 pg/ml), mothers (768 +/- 261 pg/ml), and adult volunteers (681 +/- 140 pg/ml) (P < 0.001), whereas the fetal level of FGF23 was lower than levels in the other subjects. The levels of soluble alpha-Klotho were negatively correlated with those of FGF23 in cord blood. Immunohistochemistry demonstrated that alpha-Klotho was predominantly expressed in syncytiotrophoblasts in normal term placenta.Conclusion: Levels of soluble alpha-Klotho are markedly elevated in cord blood and might be useful as a biomarker for mineral metabolism in the fetus. (J Clin Endocrinol Metab 96: E943-E947, 2011)