Application of monoclonal antibodies in quantifying fungal growth dynamics during aerobic spoilage of silage.
Application of monoclonal antibodies in quantifying fungal growth dynamics during aerobic spoilage of silage.
复制标题
单克隆抗体在量化青贮饲料有氧腐败过程中真菌生长动态中的应用。
DOI：
10.1111/1751-7915.13552
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发表时间：
2020
影响因子：
5.7
通讯作者：
Le Cocq K
中科院分区：
文献类型：
--
作者：
Le Cocq K
丝状真菌在氧气进入青贮后的增殖是干物质损失的重要原因，造成了显著的浪费。此外，某些丝状真菌产生的真菌毒素通过真菌中毒对动物健康构成风险。通过测量麦角甾醇含量、菌落形成单位或温度升高对青贮中真菌生长进行定量评估，由于在代表真菌生物量或能够识别特定属方面存在缺陷，因此在代表好氧变质过程中真菌生长动态方面受到限制。在这里，我们进行了受控环境有氧暴露实验，以测试单克隆抗体为基础的酶联免疫吸附试验（ELISA）检测真菌生物量在6种青贮样品中的增殖的有效性。我们将其与传统上用于此类实验和农场的温度进行了比较，以检测有氧变质。此外，我们还量化了麦角甾醇，这是真菌生物量的第二个标志。经过8天的有氧暴露后，麦角甾醇和ELISA方法表明，其中一个样品的真菌生物量增加，16天后观察到温度升高。与Pearson相关系数的比较表明，温度与麦角甾醇和真菌生物量的两个指标呈正相关。这项工作表明，该技术有可能被用作保存饲料中微生物降解的指标。因此，如果将其发展为一种农场技术，它可以为农民的饲料管理决策提供信息，以减少干物质损失，提高资源和养分效率，并降低对动物健康的风险。
Proliferation of filamentous fungi following ingress of oxygen to silage is an important cause of dry matter losses, resulting in significant waste. In addition, the production of mycotoxins by some filamentous fungi poses a risk to animal health through mycotoxicosis. Quantitative assessment of fungal growth in silage, through measurement of ergosterol content, colony‐forming units or temperature increase is limiting in representing fungal growth dynamics during aerobic spoilage due to being deficient in either representing fungal biomass or being able to identify specific genera. Here, we conducted a controlled environment aerobic exposure experiment to test the efficacy of a monoclonal antibody‐based enzyme‐linked immunosorbent assay (ELISA) to detect the proliferation of fungal biomass in six silage samples. We compared this to temperature which has been traditionally deployed in such experiments and on‐farm to detect aerobic deterioration. In addition, we quantified ergosterol, a second marker of fungal biomass. After 8 days post‐aerobic exposure, the ergosterol and ELISA methods indicated an increase in fungal biomass in one of the samples with a temperature increase observed after 16 days. A comparison of the methods with Pearson's correlation coefficient showed a positive association between temperature and ergosterol and both markers of fungal biomass. This work indicates that the technology has potential to be used as an indicator of microbial degradation in preserved forage. Consequently, if it developed as an on‐farm technique, this could inform forage management decisions made by farmers, with the goal of decreasing dry matter losses, improving resource and nutrient efficiency and reducing risks to animal health.