The primary target cells of the high-risk cottontail rabbit papillomavirus colocalize with hair follicle stem cells.
The primary target cells of the high-risk cottontail rabbit papillomavirus colocalize with hair follicle stem cells.
复制标题
高危棉尾兔乳头瘤病毒的主要靶细胞与毛囊干细胞共存。
DOI：
10.1128/jvi.70.3.1912-1922.1996
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Iftner,T
中科院分区：
文献类型：
--
作者：
Schmitt,A;Rochat,A;Zeltner,R;Borenstein,L;Barrandon,Y;Wettstein,FO;Iftner,T
乳头瘤病毒是一种小的DNA肿瘤病毒，其生命周期与复层上皮的分化密切相关。皮肤上皮层的感染可能保持潜伏状态，也可能导致良性肿瘤的发展。然而，某些不同的乳头瘤病毒类型引起的病变具有进展为癌的高风险，并且已经做出了广泛的努力来理解这一过程。对持续感染和乳头状瘤发展过程中的初始事件知之甚少。虽然人们普遍认为乳头状瘤的生长需要上皮基底层细胞的感染，但仍不清楚哪些细胞执行这一任务。我们分析了原位杂交活检样品采取了不同的时间点感染后的家兔与棉尾兔乳头状瘤病毒。在11天后取的活检样品中以低频率检测到的阳性细胞主要表达高水平的E6和E7 mRNA，并且定位于外上皮根鞘和毛囊球中。克隆分析的角质形成细胞分离的不同亚片段的个别兔毛囊表现出明确的colocalization的棉尾兔乳头瘤病毒的mRNA阳性细胞与克隆形成细胞的毛囊。这些数据表明，有能力建立乳头状瘤生长的细胞代表了毛囊中角质形成细胞的亚群，具有上皮干细胞的预期特性。
Papillomaviruses are small DNA tumor viruses with a life cycle inseparably linked to the differentiation of the pluristratified epithelium. The infection of epithelial layers of the skin may remain latent or may result in the development of benign tumors. A certain number of distinct papillomavirus types, however, cause lesions which have a high risk of progression into carcinomas, and extensive efforts have been made to understand this process. comparatively little is known about the initial events during the establishment of a persistent infection and papilloma development. Although it is generally accepted that the growth of a papilloma requires the infection of cells in the basal layer of the epithelium, it remains unknown which cells perform this task. We have analyzed by in situ hybridization biopsy samples taken at various time points after infection of domestic rabbits with cottontail rabbit papillomavirus. The positive cells detected at a low frequency in biopsy samples taken after 11 days predominantly expressed high levels of E6 and E7 mRNA and were localized in the outer epithelial root sheath and in the bulbs of hair follicles. A clonal analysis of keratinocytes isolated from different subfragments of individual rabbit hair follicles demonstrated a clear colocalization of cottontail rabbit papillomavirus mRNA-positive cells with clonogenic cells in hair follicles. These data suggest that the cells competent to establish papillomatous growth represent a subpopulation of keratinocytes in hair follicles with properties expected of epithelial stem cells.