Characterization of Bioeffects on Endothelial Cells under Acoustic Droplet Vaporization.
Characterization of Bioeffects on Endothelial Cells under Acoustic Droplet Vaporization.
复制标题
DOI：
10.1016/j.ultrasmedbio.2015.07.019
复制
发表时间：
2015-12
影响因子：
2.9
通讯作者：
Bull JL
中科院分区：
文献类型：
--
作者：
Seda R;Li DS;Fowlkes JB;Bull JL
气体栓塞术是通过声学微滴汽化(ADV)局部汽化微滴实现的，这种微滴汽化会产生足够大的气泡，以阻断流向肿瘤的血流。作为血管衬里的内皮细胞可能会受到这些汽化事件的影响，导致细胞损伤和细胞死亡。使用3.5 MHz换能器和十二氟戊烷液滴将理想的内皮细胞单层暴露在ADV中。治疗包括从2到8兆帕(稀疏)的辐射压力和从4到16个输入周期的脉冲长度。产生的气泡云直接依赖于压力，而不是脉冲长度。细胞损伤随着气泡云大小的增加而增加，但仅限于气泡云区域。这些结果表明，内皮附近的汽化可能会影响血管壁，这种影响可能是有害的，也可能是有益的，具体取决于预期的整体治疗应用。
Gas embolotherapy is achieved by locally vaporizing microdroplets through acoustic droplet vaporization (ADV), which result in bubbles that are large enough to occlude blood flow directed to tumors. Endothelial cells, lining blood vessels, can be affected by these vaporization events leading to cell injury and cell death. An idealized monolayer of endothelial cells was exposed to ADV using a 3.5 MHz transducer and dodecafluoropentane droplets. Treatments included insonation pressures that varied from 2 to 8 MPa (rarefactional), and pulse lengths that varied from 4 to 16 input cycles. The generated bubble cloud was directly dependent on pressure, but not on pulse length. Cellular damage increased with increasing bubble cloud size, but was limited to the bubble cloud area. These results suggest that vaporization near the endothelium may impact the vessel wall, an effect that could be either deleterious or beneficial depending on the intended overall therapeutic application.