A Novel Nanoparticle Mediated Selective Inner Retinal Photocoagulation for Diseases of the Inner Retina.
A Novel Nanoparticle Mediated Selective Inner Retinal Photocoagulation for Diseases of the Inner Retina.
复制标题
DOI：
10.1109/tnb.2017.2741490
复制
发表时间：
2017-10
影响因子：
3.9
通讯作者：
Balasubramanian M
中科院分区：
文献类型：
--
作者：
Singh R;Rajaraman S;Balasubramanian M
提出了一种新的纳米粒子介导的方法，用于视网膜内层的激光光凝，以实现组织选择性治疗。使用Bouguer-Beer-Lambert吸收定律对527 nm、577 nm和810 nm激光的传输、玻璃体、视网膜、RPE、脉络膜和巩膜中的热沉积和最终热损伤进行建模，并使用有限体积法进行数值求解。纳米粒子的设计使用米氏散射理论。将使用金纳米球和金-二氧化硅纳米壳的新的光凝策略的性能与没有纳米颗粒的常规方法的性能进行比较。为了实验验证，用金纳米球注入离体猪眼的玻璃体腔。在纳米颗粒扩散约6小时后，用绿色激光照射猪视网膜，并使用光谱域光学相干断层扫描（Spectralis SD-OCT，Heidelberg Engineering）同时成像。我们的计算模型预测，当注入纳米颗粒时，峰值温度从RPE到视网膜内部区域的显著空间偏移。对于527 nm激光，在约14 ms内实现了视网膜中部位置的视网膜动脉热损伤，从而与不使用纳米颗粒的治疗相比，将照射持续时间减少了约30 ms。在注入金纳米球的离体猪眼中，SD-OCT视网膜图像显示由于激光光凝，RPE处的热损伤和膨胀较低。纳米粒子注入的激光光凝策略提供了一种选择性的内视网膜热损伤，激光功率和激光曝光时间显着减少。所提出的治疗策略显示了高效和高选择性视网膜内激光治疗的可能性。
A novel nanoparticle mediated methodology for laser photocoagulation of the inner retina to achieve tissue selective treatment is presented. Transport of 527 nm, 577 nm, and 810 nm laser, heat deposition and eventual thermal damage in vitreous, retina, RPE, choroid and sclera were modelled using Bouguer-Beer-Lambert law of absorption and solved numerically using the finite volume method. Nanoparticles were designed using Mie theory of scattering. Performance of the new photocoagulation strategy using gold nanospheres and gold-silica nanoshells was compared to that of conventional methods without nanoparticles. For experimental validation, vitreous cavity of ex vivo porcine eyes was infused with gold nanospheres. After ~6 hours of nanoparticle diffusion, the porcine retina was irradiated with a green laser and imaged simultaneously using a spectral domain optical coherence tomography (Spectralis SD-OCT, Heidelberg Engineering). Our computational model predicted a significant spatial shift in the peak temperature from RPE to the inner retinal region when infused with nanoparticles. Arrhenius thermal damage in the mid-retinal location was achieved in ~14 ms for 527 nm laser thereby reducing the irradiation duration by ~30 ms compared to treatment without nanoparticles. In ex vivo porcine eyes infused with gold nanospheres, SD-OCT retinal images revealed a lower thermal damage and expansion at RPE due to laser photocoagulation. Nanoparticle infused laser photocoagulation strategy provided a selective inner retinal thermal damage with significant decrease in laser power and laser exposure time. The proposed treatment strategy shows possibilities for an efficient and highly selective inner retinal laser treatment.