Molecular taxonomy of Colletotrichum species causing anthracnose on the Malvaceae
Molecular taxonomy of Colletotrichum species causing anthracnose on the Malvaceae
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DOI：
10.1094/phyto-86-1076
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发表时间：
1996-10-01
期刊：
影响因子：
3.2
通讯作者：
TeBeest, DO
中科院分区：
文献类型：
--
作者：
Bailey, JA;Nash, C;TeBeest, DO
本文对炭疽菌（Colletotrichum（C. gossypii;棉假丝酵母C.棉原变种cephalosporioides; C.胶孢菌属sp. malearia，包括BioMal;和C.从棉花、Lavatera trimestris、Malva pusilla和Sida spinosa中分离纯化了10种植物提取物。对于这些菌株的代表性样品，分生孢子的形态和分化，其亲和凝集素羊蹄甲凝集素（BPA）和单克隆抗体（UB 20），其感染菌丝的性质进行了评估与rDNA序列数据的分析。结果表明，所有的L. trimestris，M. pusilla和S. spinosa，包括BioMal和其他几个C.胶孢菌属sp. malignant，是C.圆形聚集种。所有菌株均产生直圆柱形分生孢子，萌发后20天仍保持无菌状态。这些分离物与其他类型的C.通过检测它们的初始感染过程和通过它们的rDNA的限制性分析，证实了Orbiculare种聚集体。棉花分离物的分析表明，它们彼此相似，并与几种类型的C。胶孢菌因此，将C. gossypii为一个与棉蚜C.胶孢菌C.本文讨论了在寄主特异性的分子基础上研究圆形种聚集的可能性。
The taxonomic status of isolates of Colletotrichum (C. gossypii; C. gossypii var. cephalosporioides; C. gloeosporioides f. sp. malvae, including BioMal; and C. malvarum) from cotton, Lavatera trimestris, Malva pusilla, and Sida spinosa was studied. For a representative sample of these isolates, conidial morphology and differentiation, their affinity for the lectin Bauhinia purpurea agglutinin (BPA) and a monoclonal antibody (UB 20), and the nature of their infection hyphae were assessed in association with an analysis of rDNA sequence data. The results revealed that all the isolates from L. trimestris, M. pusilla, and S. spinosa, including BioMal and several other samples of C. gloeosporioides f. sp. malvae, were forms of the C. orbiculare aggregate species. All these isolates produced straight-cylindrical conidia that hound BPA and UB 20 nd remained aseptate after germination. The similarity of these isolates to other forms of the C. orbiculare species aggregate was confirmed by examination of their initial infection process and by restriction analysis of their rDNA. Analysis of isolates from cotton showed that they were similar to each other and to several forms of C. gloeosporioides. Thus, it is probably not appropriate to regard C. gossypii as a species distinct from C. gloeosporioides. The suitability of the C. orbiculare species aggregate for studies on the molecular basis of host specificity is discussed.