Kinetic sculpting of the seven stripes of the Drosophila even-skipped gene.
Kinetic sculpting of the seven stripes of the Drosophila even-skipped gene.
复制标题
果蝇的七条条纹的动态雕刻甚至跳过了基因。
DOI：
10.7554/elife.61635
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发表时间：
2020-12-10
期刊：
影响因子：
7.7
通讯作者：
Eisen MB
中科院分区：
文献类型：
--
作者：
Berrocal A;Lammers NC;Garcia HG;Eisen MB
我们利用实时成像技术在单细胞和高时间分辨率下可视化黑腹果蝇甚至跳过基因的转录动态，并开发了工具来表征和可视化转录爆发随时间和空间的变化。我们发现，尽管是由五个增强子的独立活动产生的，但即使是跳过的条纹也是由相同的动力学现象雕刻的：脉冲频率和振幅的耦合增加。通过跟踪单个细胞核的位置和活动，我们发现条纹运动是由从后到前条纹侧的爆发核交换驱动的。我们的工作为在空间和时间上解剖模式形成提供了一个概念、理论和计算框架，并揭示了单个细胞核的协调转录活性如何塑造复杂的发育模式。
We used live imaging to visualize the transcriptional dynamics of the Drosophila melanogaster even-skipped gene at single-cell and high-temporal resolution as its seven stripe expression pattern forms, and developed tools to characterize and visualize how transcriptional bursting varies over time and space. We find that despite being created by the independent activity of five enhancers, even-skipped stripes are sculpted by the same kinetic phenomena: a coupled increase of burst frequency and amplitude. By tracking the position and activity of individual nuclei, we show that stripe movement is driven by the exchange of bursting nuclei from the posterior to anterior stripe flanks. Our work provides a conceptual, theoretical and computational framework for dissecting pattern formation in space and time, and reveals how the coordinated transcriptional activity of individual nuclei shapes complex developmental patterns.