Cannabinoid CB1 receptors are localized primarily on cholecystokinin-containing GABAergic interneurons in the rat hippocampal formation.
Cannabinoid CB1 receptors are localized primarily on cholecystokinin-containing GABAergic interneurons in the rat hippocampal formation.
复制标题
大麻素 CB1 受体主要位于大鼠海马结构中含有胆囊收缩素的 GABA 能中间神经元上。
DOI：
10.1016/s0306-4522(99)00086-x
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发表时间：
1999
期刊：
影响因子：
3.3
通讯作者：
Walker,JM
中科院分区：
文献类型：
--
作者：
Tsou,K;Mackie,K;Sañudo-Peña,MC;Walker,JM
应用免疫组织化学双标记技术和共聚焦显微镜技术研究了大麻素CB1受体在大鼠海马结构GABA能中间神经元上的分布。几乎所有CB1免疫反应神经元（95%）都是GABA能神经元。CB1荧光呈点状。相反，GABA荧光分布均匀，这表明虽然CB1受体和GABA存在于相同的细胞中，但它们并不位于相同的亚细胞区室中。虽然几乎所有的CB1神经元都是GABA能的，但许多GABA能神经元不含CB1受体。海马结构中的GABA能中间神经元可以使用细胞标记物如神经肽和钙结合蛋白进一步分为具有不同连接和功能的亚群。CB1受体与胆囊收缩素高度共定位，与钙视黄蛋白和钙结合蛋白部分共定位，但不与小清蛋白。这表明，大麻素可以调节在索马上的突触和在主神经元的近端树突上的突触处的GABA能神经传递，以及在其他GABA能中间神经元上的突触处。
Localization of cannabinoid CB1 receptors on GABAergic interneurons in the rat hippocampal formation was studied by double-labeling immunohistochemistry with confocal microscopy. Virtually all CB1-immunoreactive neurons (95%) are GABAergic. CB1 fluorescence showed a punctate pattern. In contrast, the GABA fluorescence was distributed homogeneously, suggesting that while CB1 receptors and GABA exist in the same cells they are not localized in the same subcellular compartments. Although virtually all CB1 neurons were GABAergic, many GABAergic neurons did not contain CB1 receptors. GABAergic interneurons in the hippocampal formation can be further divided into subpopulations with distinct connections and functions, using cell markers such as neuropeptides and calcium binding proteins. CB1 receptors were highly co-localized with cholecystokinin and partially co-localized with calretinin and calbindin, but not with parvalbumin. This suggests that cannabinoids may modulate GABAergic neurotransmission at the synapses on the soma and at synapses on the proximal dendrites of the principal neurons, as well as at synapses on other GABAergic interneurons.