An improved method of transcutaneous cisterna magna puncture for cerebrospinal fluid sampling in rats
An improved method of transcutaneous cisterna magna puncture for cerebrospinal fluid sampling in rats
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DOI：
10.1016/j.jneumeth.2012.09.013
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发表时间：
2012-11-15
影响因子：
3
通讯作者：
Surendran, Narayanan
中科院分区：
文献类型：
--
作者：
Mahat, Mahamad Yunnus A.;Ahamed, N. Fakrudeen Ali;Surendran, Narayanan
A simple, reproducible and chronic technique of cerebrospinal fluid (CSF) collection in rats was developed by direct cisterna magna (CM) puncture utilizing stereotaxic apparatus. CSF collection apparatus was constructed using 1 mL syringe, silicone tubing, 21G disposable needle and water. Animal was placed on an elevated platform over stereotaxic apparatus base and puncture site was identified with the aid of stereotaxic co-ordinates. The volume of CSF collected varied from 100 to 180 mu L with mean CSF volume of 150 mu L Neurological deficits were recorded according to the modified Bederson's scoring system 24 h post CSF collection and differential cell count in CSF samples was performed. Animals continued to be normal with regular feed intake and gained body weight (similar to 24%) even after repeated sampling for four weeks and showed no severe neurological deficits (mean Bederson score
