Label-free imaging and biochemical characterization of bovine sperm cells.
Label-free imaging and biochemical characterization of bovine sperm cells.
复制标题
DOI：
10.3390/bios5020141
复制
发表时间：
2015-04-01
期刊：
影响因子：
--
通讯作者：
De Luca AC
中科院分区：
文献类型：
--
作者：
Ferrara MA;Di Caprio G;Managò S;De Angelis A;Sirleto L;Coppola G;De Luca AC
在人工授精的准备过程中，完全无标记的形态和生化特征是选择精子所必需的。为了研究这些基本参数，我们利用了两种很有吸引力的技术：数字全息和拉曼光谱。卫生学为非侵入性、定量和不需要染色或标记的细胞和组织的形态方面提供了新的机会，而遥感是一种非常专门的技术，允许对细胞成分进行空间分辨率在亚微米范围内的生化分析。本文利用这些技术对牛精子细胞的形态和生化变化进行了研究。此外，还进行了互补的DH和RS研究，以鉴定携带X和Y染色体的精子细胞。我们证明，这两种技术结合在一起是一种强大而高效的工具，可以阐明精子形态选择和性别鉴定的一些重要标准，克服了与现有方案相关的许多限制。
A full label-free morphological and biochemical characterization is desirable to select spermatozoa during preparation for artificial insemination. In order to study these fundamental parameters, we take advantage of two attractive techniques: digital holography (DH) and Raman spectroscopy (RS). DH presents new opportunities for studying morphological aspect of cells and tissues non-invasively, quantitatively and without the need for staining or tagging, while RS is a very specific technique allowing the biochemical analysis of cellular components with a spatial resolution in the sub-micrometer range. In this paper, morphological and biochemical bovine sperm cell alterations were studied using these techniques. In addition, a complementary DH and RS study was performed to identify X- and Y-chromosome-bearing sperm cells. We demonstrate that the two techniques together are a powerful and highly efficient tool elucidating some important criterions for sperm morphological selection and sex-identification, overcoming many of the limitations associated with existing protocols.