Cross-protection between antigenically distinct H1N1 swine influenza viruses from Europe and North America.
Cross-protection between antigenically distinct H1N1 swine influenza viruses from Europe and North America.
复制标题
来自欧洲和北美的抗原不同的 H1N1 猪流感病毒之间的交叉保护。
DOI：
10.1111/j.1750-2659.2010.00164.x
复制
发表时间：
2011-03
影响因子：
4.4
通讯作者：
Van Reeth K
中科院分区：
文献类型：
--
作者：
De Vleeschauwer AR;Van Poucke SG;Karasin AI;Olsen CW;Van Reeth K
请引用本文：De Vleeschauwer 等人。 (2011) 来自欧洲和北美的抗原性不同的 H1N1 猪流感病毒之间的交叉保护。流感和其他呼吸道病毒 5(2), 115–122。 背景 类似禽类的 H1N1 猪流感病毒 (SIV) 在西欧猪群中流行。该病毒在抗原上与北美的 H1N1 SIV 不同，后者具有经典猪病毒谱系 H1 血凝素，2009 年大流行 (H1N1) 病毒也是如此。然而，这种抗原差异对于猪之间交叉保护的意义仍然未知。 目标 我们检查了 4 周前感染欧洲禽类 SIV [A/swine/Belgium/1/98 (sw/B/98)] 的猪对北美三重重组 H1N1 SIV [A/swine/Iowa/H04YS2/04 (sw/IA/04)] 感染的保护作用。我们还检查了两种 SIV 与 2009 年大流行 (H1N1) 病毒之间的遗传关系和血清学交叉反应性 [A/California/04/09 (Calif/09)]。 结果 鼻内接种 sw/IA/04 后，所有先前未感染的对照猪均表现出鼻腔病毒排泄、攻击后 4 天 (DPCh) 整个呼吸道中的高病毒滴度以及肉眼可见的肺部病变。大多数先前感染过 sw/B/98 的猪的鼻拭子和呼吸道组织中的 sw/IA/04 检测呈阴性，且没有肺部病变。在攻击时，这些猪具有低水平的针对 sw/IA/04 的交叉反应性病毒中和和神经氨酸酶抑制 (NI) 抗体，但没有血凝抑制抗体。当针对 Calif/09 进行测试时，它们显示出相似的抗体谱，但针对 Calif/09 的 NI 抗体滴度高于针对 sw/IA/04 的 NI 抗体滴度，反映出 sw/B/98 神经氨酸酶与 Calif/09 具有更高的遗传同源性。 结论 我们的数据表明，感染欧洲禽类 H1N1 SIV 诱导的免疫力可以为猪提供针对北美 H1N1 SIV 和经典 H1 病毒的保护，并且表明对 2009 年大流行 (H1N1) 病毒具有交叉保护作用。
Please cite this paper as: De Vleeschauwer et al. (2011) Cross‐protection between antigenically distinct H1N1 swine influenza viruses from Europe and North America. Influenza and Other Respiratory Viruses 5(2), 115–122. Background An avian‐like H1N1 swine influenza virus (SIV) is enzootic in swine populations of Western Europe. The virus is antigenically distinct from H1N1 SIVs in North America that have a classical swine virus‐lineage H1 hemagglutinin, as does the pandemic (H1N1) 2009 virus. However, the significance of this antigenic difference for cross‐protection among pigs remains unknown. Objectives We examined protection against infection with a North American triple reassortant H1N1 SIV [A/swine/Iowa/H04YS2/04 (sw/IA/04)] in pigs infected with a European avian‐like SIV [A/swine/Belgium/1/98 (sw/B/98)] 4 weeks earlier. We also examined the genetic relationships and serologic cross‐reactivity between both SIVs and with a pandemic (H1N1) 2009 virus [A/California/04/09 (Calif/09)]. Results After intranasal inoculation with sw/IA/04, all previously uninfected control pigs showed nasal virus excretion, high virus titers in the entire respiratory tract at 4 days post‐challenge (DPCh) and macroscopic lung lesions. Most pigs previously infected with sw/B/98 tested negative for sw/IA/04 in nasal swabs and respiratory tissues, and none had lung lesions. At challenge, these pigs had low levels of cross‐reactive virus neutralizing and neuraminidase inhibiting (NI) antibodies to sw/IA/04, but no hemagglutination‐inhibiting antibodies. They showed similar antibody profiles when tested against Calif/09, but NI antibody titers were higher against Calif/09 than sw/IA/04, reflecting the higher genetic homology of the sw/B/98 neuraminidase with Calif/09. Conclusions Our data indicate that immunity induced by infection with European avian‐like H1N1 SIV affords protection for pigs against North American H1N1 SIVs with a classical H1, and they suggest cross‐protection against the pandemic (H1N1) 2009 virus.