Anti-high mobility group box 1 (anti-HMGB1) antibodies are not related to the occurrence of cutaneous lesions in systemic lupus erythematosus
Anti-high mobility group box 1 (anti-HMGB1) antibodies are not related to the occurrence of cutaneous lesions in systemic lupus erythematosus
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DOI：
10.3109/03009742.2014.928946
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发表时间：
2015-01-01
影响因子：
2.1
通讯作者：
Wang, L.
中科院分区：
文献类型：
--
作者：
Tan, G.;Zhu, N.;Wang, L.
目的：探讨血清抗高迁移率族蛋白1（抗HMGB1）抗体是否与系统性红斑狼疮（SLE）皮损的发生有关。方法：本研究纳入21名有皮损的SLE患者、18名无皮损的SLE患者和22名健康对照者。分别通过蛋白质印迹和酶联免疫吸附测定（ELISA）测量抗HMGB1-IgG和-IgM的存在和血清水平。通过免疫荧光染色观察皮肤中沉积的 HMGB1 表达和血清抗体。结果：使用蛋白质印迹分析，我们在 21 名有皮损的 SLE 患者中的 13 名和 18 名无皮损的 SLE 患者中检测到了 11 名抗 HMGB1-IgG 抗体（p > 0.05）。通过 ELISA 测量的抗 HMGB1-IgG 血清水平在两组 SLE 患者之间也具有可比性 (p > 0.05)，但患者中的水平高于健康对照 (p < 0.05)。血清抗 HMGB1-IgM 抗体也发现了类似的结果。 HMGB1在狼疮皮损处积累于角质层下，不与IgG或IgM形成免疫复合物，主要沿表皮-真皮交界处观察到。此外，关节炎患者组的血清抗HMGB1-IgM水平高于无关节炎患者。结论：根据本研究的结果，抗HMGB1抗体不太可能在SLE皮肤病变的发生过程中发挥作用。
Objectives: To investigate whether serum anti-high mobility group box 1 (anti-HMGB1) antibodies are related to the development of skin lesions in systemic lupus erythematosus (SLE).Method: This study involved 21 SLE patients with skin lesions, 18 without skin lesions, and 22 healthy controls. The presence and serum levels of anti-HMGB1-IgG and -IgM were measured by western blot and enzyme-linked immunosorbent assay (ELISA), respectively. HMGB1 expression and serum antibodies deposited in the skin were visualized by immunofluorescence staining.Results: Using western blot analysis, we detected anti-HMGB1-IgG antibodies in 13 out of 21 SLE patients with skin lesions and 11 out of 18 SLE patients without skin lesions (p > 0.05). Serum levels of anti-HMGB1-IgG measured by ELISA were also comparable between the two groups of SLE patients (p > 0.05) but were higher in patients than in healthy controls (p < 0.05). Similar results were found with serum anti-HMGB1-IgM antibodies. HMGB1 accumulated under the stratum corneum in lupus cutaneous lesions without forming immune complexes with IgG or IgM, which were mainly observed along the epidermal-dermal junction. Furthermore, serum anti-HMGB1-IgM was higher in the group of patients with arthritis than in those without arthritis.Conclusions: Based on the findings of the current study, it is unlikely that anti-HMGB1 antibodies play a role in the development of SLE cutaneous lesions.