Opioid receptor gene expression in the rat brain during ontogeny, with special reference to the mesostriatal system: an in situ hybridization study
Opioid receptor gene expression in the rat brain during ontogeny, with special reference to the mesostriatal system: an in situ hybridization study
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DOI：
10.1016/s0165-3806(98)00082-0
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发表时间：
1998-08-08
期刊：
影响因子：
--
通讯作者：
Le Moine, C
中科院分区：
文献类型：
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作者：
Georges, F;Normand, E;Le Moine, C
三种主要类型的阿片受体μ、δ和κ存在于中枢神经系统和外周。本研究采用原位杂交技术研究了大鼠出生前后脑内μ、δ、κ阿片受体mRNA的表达，尤其是纹状体系统的表达。G13时，端脑可检测到μ和κ阿片受体mRNA，纹状体神经上皮和皮质板可检测到μ阿片受体mRNA，腐蚀裂可检测到κ阿片受体mRNA。到G15，κ-阿片受体mRNA可检测到的背侧纹状体，在黑质和腹侧被盖区，多巴胺能终末纤维上的相应受体的早期表达。对于纹状体中的μ-阿片受体mRNA，在G20出现斑块。δ-阿片受体mRNA在G21时首次在包括中脑核和背侧纹状体的许多区域中被检测到。在P8，δ-阿片受体mRNA检测到大尺寸的细胞的纹状体，可能是胆碱能，这表明可能的调制阿片类物质的纹状体胆碱能神经元。我们的研究结果表明，CL和κ阿片受体mRNA（G13）的早期出现和δ阿片受体mRNA（G21）在大脑中的相对较晚的发展。我们还显示了一个独特的模式，μ，δ和κ-阿片受体mRNA的表达在中纹状体系统的发展过程中。(C)1998 Elsevier Science B. V.保留所有权利。
The three main types of opioid receptors mu, delta and kappa are found in the central nervous system and periphery. In situ hybridization study was undertaken to determine the expression of mu, delta, kappa-opioid receptors mRNAs in the brain during pre- and postnatal development, especially in the mesostriatal system. By G13, mu and kappa-opioid receptor mRNA were detectable in the telencephalon; mu-opioid receptor mRNA was found in the striatal neuroepithelium and cortical plate and kappa-opioid receptor mRNA in the corroidal fissure. By G15, kappa-opioid receptor mRNA was detectable in the nucleus accumbens and dorsal striatum, and in the substantia nigra and ventral tegmental area, suggesting an early expression of the corresponding receptor on dopaminergic terminal fibers. For the mu-opioid receptor mRNA in the striatum, patches appeared at G20. delta-Opioid receptor mRNA was first detected at G21, in many areas including the accumbens nucleus and the dorsal striatum. At P8, delta-opioid receptor mRNA was detected in large-sized cells of the striatum, possibly cholinergic, suggesting a possible modulation by opioids of the striatal cholinergic neurons. Our results demonstrate the early appearance of CL and kappa-opioid receptor mRNA (G13) and the relatively late development of delta-opioid receptor mRNA (G21) in the brain. We also show a distinct pattern of expression for mu, delta and kappa-opioid receptor mRNAs in the mesostriatal system during the development. (C) 1998 Elsevier Science B.V. All rights reserved.