Over-Expression of OsHOX24 Confers Enhanced Susceptibility to Abiotic Stresses in Transgenic Rice via Modulating Stress-Responsive Gene Expression.
Over-Expression of OsHOX24 Confers Enhanced Susceptibility to Abiotic Stresses in Transgenic Rice via Modulating Stress-Responsive Gene Expression.
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DOI：
10.3389/fpls.2017.00628
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发表时间：
2017
影响因子：
5.6
通讯作者：
Jain M
中科院分区：
文献类型：
--
作者：
Bhattacharjee A;Sharma R;Jain M
同源框转录因子在植物发育和非生物胁迫反应中起着重要作用。本研究以水稻为材料，构建了高表达胁迫响应基因OsHOX24（水稻同源结构域亮氨酸拉链I亚家族成员）的转基因水稻，并分析了其在不同发育阶段对各种非生物胁迫的响应。在种子萌发阶段，与野生型（WT）相比，过表达OsHOX24的转基因水稻表现出对非生物胁迫条件和脱落酸的增强的敏感性。OsHOX24过表达水稻幼苗在盐和干燥胁迫（DS）条件下表现出根和茎的生长降低。各种生理和表型测定证实，在水稻发育的成熟和生殖阶段，转基因水稻对非生物胁迫的敏感性也高于野生型。全球基因表达谱显示，控制和DS条件下的转基因植物中的几个基因的差异调节。这些差异表达基因除了参与碳水化合物、核酸和脂类代谢过程以及对非生物胁迫和激素的应答外，还参与转录调控活动。综上所述，我们的研究结果强调了OsHOX24通过调节水稻胁迫响应基因的表达来调节非生物胁迫响应的作用。
Homeobox transcription factors play critical roles in plant development and abiotic stress responses. In the present study, we raised rice transgenics over-expressing stress-responsive OsHOX24 gene (rice homeodomain-leucine zipper I sub-family member) and analyzed their response to various abiotic stresses at different stages of development. At the seed germination stage, rice transgenics over-expressing OsHOX24 exhibited enhanced sensitivity to abiotic stress conditions and abscisic acid as compared to wild-type (WT). OsHOX24 over-expression rice seedlings showed reduced root and shoot growth under salinity and desiccation stress (DS) conditions. Various physiological and phenotypic assays confirmed higher susceptibility of rice transgenics toward abiotic stresses as compared to WT at mature and reproductive stages of rice development too. Global gene expression profiling revealed differential regulation of several genes in the transgenic plants under control and DS conditions. Many of these differentially expressed genes were found to be involved in transcriptional regulatory activities, besides carbohydrate, nucleic acid and lipid metabolic processes and response to abiotic stress and hormones. Taken together, our findings highlighted the role of OsHOX24 in regulation of abiotic stress responses via modulating the expression of stress-responsive genes in rice.