Molecular and biomarker analyses of salivary duct carcinomas: comparison with mammary duct carcinoma.
Molecular and biomarker analyses of salivary duct carcinomas: comparison with mammary duct carcinoma.
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唾液管癌的分子和生物标志物分析：与乳腺导管癌的比较。
DOI：
10.3892/ijo.19.4.865
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发表时间：
2001
影响因子：
5.2
通讯作者：
A. El
中科院分区：
文献类型：
--
作者：
M. Hoang;D. Callender;J. S. Sola Gallego;Z. Huang;N. Sneige;M. Luna;J. Batsakis;A. El
摘要涎腺导管癌是一种罕见的高度侵袭性肿瘤，其组织学特征与乳腺浸润性导管癌相似。与SDC相反，对IDC进行了广泛的分子研究，并鉴定了某些生物标志物。为了研究SDC的潜在分子和生物学特征，我们使用染色体臂6 q、16 q、17 p和17 q上的微卫星标记、DNA流式细胞术以及雄激素受体（AR）和p53表达的免疫组化染色对28例这些肿瘤进行了分子分析。与24例IDC病例进行比较。我们的研究结果表明，一般相似的等位基因改变，升高p53和雄激素受体的表达，和高频率的DNA非整倍体表现在SDC和IDC。然而，在6q，17p和17q染色体位点上的某些标记的差异，观察两个实体之间。6q上的某些位点在SDC中比IDC中更频繁地发生改变，其中17 p和q臂上的位点在IDC中比SDC中更多地出现。大多数SDC具有高AR表达，而大多数IDC为AR阴性。我们的研究表明：i）SDC可能与IDC共享一些遗传改变，ii）SDC中的高AR表达可能在肿瘤进展中起作用，iii）两种实体中的p53过表达和DNA非整倍体反映了它们的侵袭性行为。
Salivary duct carcinoma (SDC) is a rare high-grade aggressive neoplasm that manifests close histologic features with invasive ductal carcinoma of the breast (IDC). In contrast to SDC, extensive molecular studies have been performed on IDC and led to the identification of certain biological markers. To investigate the underlying molecular and biologic characteristics of SDC, we performed molecular analyses using microsatellite markers on chromosomal arms 6q, 16q, 17p, and 17q, DNA flow cytometry and immunohistochemical staining for androgen receptor (AR) and p53 expression on 28 examples of these tumors in comparison to 24 IDC cases. Our results show that generally similar allelic alterations, elevated p53 and androgen receptor expressions, and high frequency of DNA aneuploidy are manifested in both SDCs and IDCs. Differences at certain markers on 6q, 17p and 17q chromosomal loci, however, were observed between the two entities. Certain loci on 6q were more frequently altered in SDC than IDC which loci on chromosomes 17p and q arms were more seen in IDCs than SDCs. The majority of SDCs had high AR expression while most of IDCs were AR negative. Our study indicates that: i) SDC may share some genetic alterations with IDC, ii) high AR expression in SDC may play a role in tumor progression, and iii) p53 overexpression and DNA aneuploidy in both entities reflect their aggressive behavior.