A genipin-crosslinked protein–polymer hybrid system for the intracellular delivery of ribonuclease A
A genipin-crosslinked protein–polymer hybrid system for the intracellular delivery of ribonuclease A
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DOI：
10.2147/ijn.s210486
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发表时间：
2019-09
影响因子：
8
通讯作者：
X. Liang;Xiuhui Tang;Jiebing Yang;Jiayuan Zhang;Haobo Han;Quanshun Li
中科院分区：
文献类型：
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作者：
X. Liang;Xiuhui Tang;Jiebing Yang;Jiayuan Zhang;Haobo Han;Quanshun Li
治疗性蛋白已广泛应用于各种疾病的治疗，而有效的载体是实现蛋白递送以获得良好治疗效力的关键。材料与方法通过格尼平介导的分子量为25000 g/mol （PEI25K）的聚乙烯亚胺与核糖核酸酶A （RNase A）即RGP的交联，构建了蛋白-聚合物杂化体系。结果RGP纳米粒子通过引入带正电荷的PEI25K，在HeLa细胞中易于国际化，从而通过切割肿瘤细胞中的RNA分子触发抗增殖作用。RGP给药后，肿瘤细胞内可见明显的红色荧光，这与吉尼平的固有特性有关。结论通过genipin介导的交联制备的蛋白质-聚合物杂化体系显示出作为体内成像和传递多种治疗蛋白的治疗平台的潜力。
Background Therapeutic proteins have been widely used in the treatment of various diseases, and effective carriers are highly required for achieving protein delivery to obtain favorable treatment potency. Materials and methods A protein–polymer hybrid system was constructed through the genipin-mediated crosslinking of polyethyleneimine with a weight-average molecular weight of 25,000 g/mol (PEI25K) and ribonuclease A (RNase A), namely RGP. Results The RGP nanoparticles were observed to be easily internationalized in HeLa cells owing to the introduction of positively charged PEI25K, thereby triggering the antiproliferative effects by cleaving RNA molecules in the tumor cells. Moreover, red fluorescence could be obviously visualized in the tumor cells after RGP delivery, which was attributed to the intrinsic characteristics of genipin. Conclusion The protein–polymer hybrid system prepared via the genipin-mediated crosslinking has exhibited potential to be used as a theranostic platform for both in vivo imaging and delivering diverse therapeutic proteins.