Repetitive sequences of the sea urchin genome. Distribution of members of specific repetitive families.
Repetitive sequences of the sea urchin genome. Distribution of members of specific repetitive families.
复制标题
海胆基因组的重复序列。
DOI：
10.1016/0022-2836(81)90332-6
复制
发表时间：
1981
影响因子：
5.6
通讯作者：
Davidson,EH
中科院分区：
文献类型：
--
作者：
Anderson,DM;Scheller,RH;Posakony,JW;McAllister,LB;Trabert,SG;Beall,C;Britten,RJ;Davidson,EH
研究了来自海胆基因组的三个重复序列家族，每个家族都通过与一到几百个核苷酸长的特定克隆探针的同源性来定义。用这些探针筛选重组 λ-海胆 DNA 文库，并选择包含这些家族基因组成员的单个重组体。进行限制性图谱、凝胶印迹和动力学分析以确定每个重复家族的组织。发现属于三个重复家族中第一个的序列元件嵌入在较长的重复序列中。这些重复序列经常以小簇形式出现。第二重复序列家族的成员也存在于长重复序列环境中，但这些重复序列通常单独出现在 DNA 的任何给定区域中。第三个重复的序列只有 200 到 300 个核苷酸长，并且通常由单拷贝 DNA 终止，尽管发现了一些与其他重复相关的例子。这三个重复序列家族构成了与 DNA 的远距离区域相关的同源序列元件组。
Three repetitive sequence families from the sea urchin genome were studied, each defined by homology with a specific cloned probe one to a few hundred nucleotides long. Recombinant λ-sea urchin DNA libraries were screened with these probes, and individual recombinants were selected that include genomic members of these families. Restriction mapping, gel blot, and kinetic analyses were carried out to determine the organization of each repeat family. Sequence elements belonging to the first of the three repeat families were found to be embedded in longer repeat sequences. These repeat sequences frequently occur in small clusters. Members of the second repeat family are also found in a long repetitive sequence environment, but these repeats usually occur singly in any given region of the DNA. The sequences of the third repeat are only 200 to 300 nucleotides long, and are generally terminated by single copy DNA, though a few examples were found associated with other repeats. These three repeat sequence families constitute sets of homologous sequence elements that relate distant regions of the DNA.