HLA-DRB1*1301 and *1302 protect against chronic hepatitis B
HLA-DRB1*1301 and *1302 protect against chronic hepatitis B
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DOI：
10.1016/s0168-8278(97)80414-x
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发表时间：
1997-03-01
影响因子：
25.7
通讯作者：
Rittner, C
中科院分区：
文献类型：
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作者：
Hohler, T;Gerken, G;Rittner, C
背景/目标：急性B型肝炎感染的结果可能受宿主因素如主要组织相容性复合体（MHC）的影响。我们研究了慢性B型肝炎患者与健康对照人群的MHC I类和II类抗原。为了证实第一项研究的结果，我们在一组从急性B型肝炎感染中自发恢复的受试者中进行了第二项研究。分析慢性B型肝炎病毒感染者和对照组中MHC I类和II类抗原的频率，采用标准微量淋巴细胞毒性试验进行MHC I类分型，采用基于聚合酶链反应的分型方法确定DRB 1和DQA 1基因型。在第一项研究中，70例慢性B型肝炎病毒感染者中有4例发现HLA-DRB 1 * 1301 - 02等位基因（5.7%），而101名健康对照者中有27名（26.7%，相对风险0.17; p = 0.001; p（corr）= 0.025），DRB 1 * 1301 - 02等位基因的这种保护作用在第二项研究中得到证实，24名患者中的8名自发清除B型肝炎病毒者DPB 1 * 1301-O2阳性率为33.3（与慢性B型肝炎受试者相比，发生慢性感染的相对风险为0.12; P = 0.004），分型证实1301和1302在慢性B型肝炎患者中的频率均降低。结论：MHC II类等位基因DRB 1 * 1301 - 02与高加索人慢性B型肝炎的保护作用有关。
Background/Aims: The outcome of acute hepatitis B infection may be influenced by host factors like the major histocompatibility complex (MHC), We have investigated MHC class I and class II antigens in patients with chronic hepatitis B compared to a healthy control population, To confirm the findings of this first study we performed a second study in a group of subjects who had spontaneously recovered from acute hepatitis B infection.Methods: Frequencies of MHC class I and class II antigens were analyzed in patients with chronic hepatitis B virus infection and in control subjects, MHC class I typing was done by standard micro-lymphocytotoxicity assays, DRB1 and DQA1 genotypes were determined by polymerase chain reaction based typing methods.Results: In the first study the class II allele HLA-DRB1*1301-02 was found in 4 of 70 subjects with chronic hepatitis B virus infection (5.7%) compared to 27 of 101 healthy controls (26.7%, relative risk 0.17; p = 0.001; p(corr) = 0.025), This protective effect of the DRB1*1301-02 allele was confirmed in the second study, Eight of 24 patients (33.3%) who cleared hepatitis B virus spontaneously were positive for DPB1*1301-O2 (relative risk of developing chronic infection compared to chronic hepatitis B subjects 0.12; p = 0.004), Subtyping confirmed that 1301 and 1302 were both decreased in frequency in patients with chronic hepatitis B.Conclusions: The MHC class II allele DRB1* 1301-02 is associated with protection from chronic hepatitis B in Caucasian patients.