In situ controllable synthesis of Schiff base networks porous polymer coatings for open-tubular capillary electrochromatography
In situ controllable synthesis of Schiff base networks porous polymer coatings for open-tubular capillary electrochromatography
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DOI：
10.1007/s00604-021-04740-1
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发表时间：
2021-02-14
影响因子：
5.7
通讯作者：
Pu, Qiaosheng
中科院分区：
文献类型：
--
作者：
Chen, Pengfei;Wu, Jing;Pu, Qiaosheng
通过毛细管内反应物的连续流动，以可控的方式原位合成了均匀的席夫碱网络（SNW）膜。研究了该毛细管的性能及其在毛细管电色谱中的应用。通过扫描电镜研究了反应单体浓度和反应时间对涂层厚度的影响。结果表明，反应条件对SNW薄膜的形貌和厚度有显著影响。膜的厚度可以通过改变反应溶液的浓度和反应时间来控制。用不同条件下包被的毛细管电泳柱对4种核苷酸进行分离，结果表明，电泳迁移时间、峰级和分离效率均发生了显著变化。该柱成功地分离了含氮杂环结构的分析物，如核苷酸、甲基咪唑异构体和β-内酰胺类抗生素。在选定的分离条件下，四种核苷酸的理论塔板数在45，237 - 104，505塔板·m（-1）范围内，分离度为1.98 - 8.07。甲基咪唑异构体的分离度为1.75。用所制备的毛细管柱测定了真实的鸡精调味料中的核苷酸，回收率在95~105%之间。
A uniform Schiff base network (SNW) film was synthesized in situ in a controllable way through continuous flow of reactants inside the capillary. The properties and application of the as-prepared capillary was investigated in capillary electrochromatography. The effects of reaction monomer concentration and reaction time on coating thickness were studied by SEM. The results show that the reaction condition has a significant influence on the morphology and thickness of the SNW films. The thickness of the film can be controlled by changing the concentration of reaction solution and reaction time. Capillaries coated under different conditions were employed to separate four nucleotides by capillary electrochromatography, which demonstrated significant variation of migration time, peak order, and separation efficiency. Analytes containing nitrogen heterocycle structures, such as nucleotides, methylimidazole isomers, and beta-lactam antibiotics, were successfully separated with the prepared open-tubular columns. Under the selected separation conditions, theoretical plate number of four nucleotides is in a range 45,237-104,505 plates.m(-1), and the resolutions are 1.98-8.07. A resolution of 1.75 is obtained for methylimidazole isomers. The nucleotides in a real sample, chicken essence seasoning, were determined using the prepared capillary column with satisfactory recoveries in the range 95 to 105%.