Epidermal growth factor influences the developmental clock regulating maturation of the fetal lung fibroblast.
Epidermal growth factor influences the developmental clock regulating maturation of the fetal lung fibroblast.
复制标题
表皮生长因子影响调节胎儿肺成纤维细胞成熟的发育时钟。
DOI：
10.1016/0167-4889(89)90097-9
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Nielsen,HC
中科院分区：
文献类型：
--
作者：
Nielsen,HC
生长因子可能在调节胎儿发育过程中器官生长和分化的有序进行中发挥重要作用。我们假设表皮生长因子（EGF）可能通过影响成纤维细胞与上皮细胞的相互作用来帮助调节胎儿肺表面活性剂合成的发育。研究了EGF （10 ng / ml）对胎儿肺成纤维细胞产生成纤维细胞肺细胞因子（FPF）能力的影响。FPF通常不由第16天的成纤维细胞产生，但在第17天仅在女性成纤维细胞中发现，然后在第18天在男性和女性中都发现。EGF促进了这种模式，女性成纤维细胞在第16天产生活性，两性成纤维细胞在第17天和第18天产生FPF活性。来自雄激素受体缺乏小鼠模型的成纤维细胞证实了EGF的作用是性别特异性的，并且与胎儿肺的发育状态有关。我们得出结论，EGF通过特定的发育阶段促进胎儿肺成纤维细胞。因此，它似乎有助于控制调节胎儿肺成熟的生物钟的时间。
Growth factors may play a significant role in regulating the orderly progression of organ growth and differentiation during fetal development. We hypothesized that epidermal growth factor (EGF) would help regulate the development of surfactant synthesis in the fetal lung by influencing fibroblast-epithelial cell interactions. The effect of EGF (10 ng per ml) on the ability of the fetal lung fibroblast to produce fibroblast pneumonocyte factor (FPF) was studied in sex-specific fibroblasts cultured from day 16, day 17 or day 18 fetal mouse lungs. FPF which is normally not produced by day 16 fibroblasts, is found only in female fibroblasts on day 17, and then in both males and females on day 18. EGF advanced this pattern such that female fibroblasts produced activity on day 16 and fibroblasts from both sexes produced FPF activity on day 17 and day 18. Fibroblasts from an androgen receptor-deficient mouse model confirmed that the effect of EGF was sex-specific and related to the state of development of the fetal lung. We conclude that EGF advances the fetal lung fubroblast through specific stages of development. It appears, therefore, to help control the timing of the clock regulating fetal lung maturation.