Effect of temperature on biofilm formation by Antarctic marine bacteria in a microfluidic device
Effect of temperature on biofilm formation by Antarctic marine bacteria in a microfluidic device
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DOI：
10.1016/j.ab.2013.10.027
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发表时间：
2014-02-01
影响因子：
2.9
通讯作者：
Lee, Chang-Soo
中科院分区：
文献类型：
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作者：
Jeong, Heon-Ho;Jeong, Seong-Geun;Lee, Chang-Soo
极地生物膜已成为一个越来越受欢迎的生物学问题，因为在极地地区的微生物中发现了新的材料和表型。各种环境因素影响微生物的功能和适应性。由于极地代表着极冷的环境，极地微生物具有与正常微生物不同的功能。因此，确定极性生物膜形成的有效温度至关重要。在这里，我们提出了一个简单的，新颖的一锅法分析温度对南极细菌生物膜形成的影响，使用微流控系统，其中产生连续的温度梯度。我们发现，一个特定的温度范围是需要的生物膜的生长。因此，这种微流体方法提供了关于极性生物膜发展的有效温度的精确信息，具有新的高通量筛选格式。(C)2013 Elsevier Inc. All rights reserved.
Polar biofilms have become an increasingly popular biological issue because new materials and phenotypes have been discovered in microorganisms in the polar region. Various environmental factors affect the functionality and adaptation of microorganisms. Because the polar region represents an extremely cold environment, polar microorganisms have a functionality different from that of normal microorganisms. Thus, determining the effective temperature for the development of polar biofilms is crucial. Here, we present a simple, novel one-pot assay for analysis of the effect of temperature on formation of Antarctic bacterial biofilm using a microfluidic system where continuous temperature gradients are generated. We find that a specific range of temperature is required for the growth of biofilms. Thus, this microfluidic approach provides precise information regarding the effective temperature for polar biofilm development with a new high-throughput screening format. (C) 2013 Elsevier Inc. All rights reserved.