Measurement of initial translational energies of peptide ions in laser desorption/ionization mass spectrometry
Measurement of initial translational energies of peptide ions in laser desorption/ionization mass spectrometry
复制标题
激光解吸/电离质谱法中肽离子初始平移能的测量
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
R. Cotter
中科院分区：
文献类型：
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作者：
Ying;R. Cotter
使用延迟离子提取、线性飞行时间质谱仪测量基质辅助紫外激光解吸/电离产生的[Arg]-加压素分子离子的初始动能分布。还测量了存在或不存在肽的烟酸基质离子的能量分布。使用红外激光解吸将这些与短杆菌肽-S 离子的动能分布进行比较。测得的加压素的分子离子动能分布比短杆菌肽-S的分子离子动能分布要宽得多，其特征是高能尾部，很可能是由于分析物离子在离开表面时被较高速度的基质离子和碎片夹带而产生的。
The initial kinetic energy distribution of [Arg]-vasopressin molecular ions generated by matrix-assisted UV laser desorption/ionization was measured using a delayed ion extraction, linear time-of-flight mass spectrometer. Energy distributions of the nicotinic acid matrix ions, with or without the presence of peptide, were also measured. These were compared with the kinetic energy distribution of gramicidin-S ions using IR laser desorption. The measured molecular ion kinetic energy distribution from vasopressin is much broader than that from gramicidin-S, and is characterized by a high-energy tail that most likely results from entrainment of anlayte ions in the higher velocity matrix ions and fragments as they leave the surface.