Transcriptomic profiling of Microplitis demolitor bracovirus reveals host, tissue and stage-specific patterns of activity
Transcriptomic profiling of Microplitis demolitor bracovirus reveals host, tissue and stage-specific patterns of activity
复制标题
DOI：
10.1099/vir.0.032680-0
复制
发表时间：
2011-09-01
影响因子：
3.8
通讯作者：
Strand, Michael R.
中科院分区：
文献类型：
--
作者：
Bitra, Kavita;Zhang, Shu;Strand, Michael R.
多脱氧核糖核酸病毒（PDVs）是一个与寄生蜂共生的DNA病毒家族。已经报道了几种PDV的特定基因或基因家族成员的转录，但是没有研究表征任何PDV分离株的扩增基因组中预测基因的整个互补序列的时空表达模式。茧蜂Microplitis demolitor携带PDV Microplitis demolitor bracovirus（MdBV），寄生幼虫阶段的拟夜蛾（Pseudoplusia）。该基因组由15个基因组片段组成，具有51个预测的ORF，编码>= 100 aa的蛋白质。这些ORF中的大多数形成四个多成员基因家族（ptp、ank、glc和egf），而其余的ORF由单拷贝（orph）基因组成。本研究采用RT-PCR和实时荧光定量PCR的方法，分析了MdBV在P. includens和M.爆破工我们的研究结果表明，大多数预测的基因在P. includens中表达。在P. includens中表达的空间模式在基因之间不同，但表达的时间模式通常是相似的，转录丰度在24和120 h之间逐渐下降。一个亚类的ptp，ank和orph基因也在成年雌性中表达，但在雄性中不表达。爆破工只有一个突变基因（ank-H4）在M.拆除者，虽然在低得多的水平比P. includens。而另一个突变基因orph-B1在M.在类似的水平，在P. includens检测到的拆除。
The polydnaviruses (PDVs) are a family of DNA viruses that are symbiotically associated with parasitoid wasps. The transcription of particular genes or gene-family members have been reported for several PDVs, but no studies have characterized the spatio-temporal patterns of expression for the entire complement of predicted genes in the encapsidated genome of any PDV isolate. The braconid wasp Microplitis demolitor carries the PDV Microplitis demolitor bracovirus (MdBV) and parasitizes larval stage Pseudoplusia (Chrysodeixis) includens. The encapsidated genome consists of 15 genomic segments with 51 predicted ORFs encoding proteins >= 100 aa. A majority of these ORFs form four multimember gene families (ptp, ank, glc and egf) while the remaining ORFs consist of single copy (orph) genes. Here we used RT-PCR and quantitative real-time PCR methods to profile the encapsidated transcriptome of MdBV in P. includens and M. demolitor. Our results indicate that most predicted genes are expressed in P. includens. Spatial patterns of expression in P. includens differed among genes, but temporal patterns of expression were generally similar, with transcript abundance progressively declining between 24 and 120 h. A subset of ptp,ank and orph genes were also expressed in adult female but not male M. demolitor. Only one encapsidated gene (ank-H4) was expressed in all life stages of M. demolitor, albeit at much lower levels than in P. includens. However, another encapsidated gene (orph-B1) was expressed in adult M. demolitor at similar levels to those detected in P. includens.